ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MSKNIKTPTN QKVLTNVAVV RMKKTGKRFE IACYKNKVVN WRNKSEKDID EVLQTHTVFS 

        70         80         90        100        110        120 
NVSKGQLSKK EELIAAFGIE DQLEICKIIL DKGDLQVSEK ERQAASDQSL KEVSQLIASM 

       130        140        150        160        170        180 
VVNPETKRPV PPSVIDKALQ EMHFSLKPNR SSKQQALDAI PKLRETLKIE RAKMKIRVAI 

       190        200        210        220        230        240 
PTKEAKSVHT KLKTLFSDVE VDDWQDGSLE MVGLIEPGSF RALDDLVRNE TKGHGRLEIL 

       250 
SLKDVVEGEL QIS 

References and documentation are available.

Number of amino acids: 253

Molecular weight: 28514.01

Theoretical pI: 9.13

Amino acid composition: 
Ala (A)  14	  5.5%
Arg (R)  12	  4.7%
Asn (N)  10	  4.0%
Asp (D)  15	  5.9%
Cys (C)   2	  0.8%
Gln (Q)  13	  5.1%
Glu (E)  22	  8.7%
Gly (G)  10	  4.0%
His (H)   4	  1.6%
Ile (I)  17	  6.7%
Leu (L)  24	  9.5%
Lys (K)  31	 12.3%
Met (M)   6	  2.4%
Phe (F)   6	  2.4%
Pro (P)   9	  3.6%
Ser (S)  20	  7.9%
Thr (T)  12	  4.7%
Trp (W)   2	  0.8%
Tyr (Y)   1	  0.4%
Val (V)  23	  9.1%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 37
Total number of positively charged residues (Arg + Lys): 43

Atomic composition:

Carbon      C	      1254
Hydrogen    H	      2091
Nitrogen    N	       353
Oxygen      O	       384
Sulfur      S	         8

Formula: C1254H2091N353O384S8
Total number of atoms: 4090

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    12615
Abs 0.1% (=1 g/l)   0.442, assuming all pairs of Cys residues form cystines


Ext. coefficient    12490
Abs 0.1% (=1 g/l)   0.438, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 44.36
This classifies the protein as unstable.



Aliphatic index: 95.10

Grand average of hydropathicity (GRAVY): -0.478