ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MSKQFVRSAK NLVKGYSSTQ VLVRNATSND NHQVSKDSLI ELAEKSYDSA DFFEIMDMLD 

        70         80         90        100        110        120 
KRLNDKGKYW RHIAKALTVI DYLIRFGSEN CVLWCRENLY IIKTLKEFRH EDDEGIDQGQ 

       130        140        150        160        170        180 
IVRVKAKELT ALLSDDERLN EERNMNIKGR NRKGRRRRGT GRSDENDDDL QRAISASRLT 

       190        200        210        220        230        240 
AEEDERRRKQ DEDYETALQL SKEEEELKRL QDLQRMQQQQ GQQQLQQPMY YDIFGNPITP 

       250        260        270        280        290        300 
EEYAQFQLQQ QQQQQQQQLQ QQPMYYDVFG NPITPEELAQ FQQQQQLQEQ QYLASMQQQQ 

       310        320        330        340        350        360 
QAMSNNPFAK SEQSSSSPKR NQLVAASSPQ QLQQQKQQEP LIQNRTGNQS MTDKYSKLNE 

       370        380        390        400        410        420 
LLATGTGIDT FGNVGEARIP AQHTKTGTFI NSQGTGYRQV SDDPNHNPFL NSQYTGLPST 

       430        440        450 
SVVPTQTGYG FGNQSQQQSQ NNGSNNRGYT LIDL 

References and documentation are available.

Number of amino acids: 454

Molecular weight: 52351.71

Theoretical pI: 5.64

Amino acid composition: 
Ala (A)  22	  4.8%
Arg (R)  29	  6.4%
Asn (N)  30	  6.6%
Asp (D)  28	  6.2%
Cys (C)   2	  0.4%
Gln (Q)  69	 15.2%
Glu (E)  31	  6.8%
Gly (G)  26	  5.7%
His (H)   5	  1.1%
Ile (I)  19	  4.2%
Leu (L)  38	  8.4%
Lys (K)  24	  5.3%
Met (M)  10	  2.2%
Phe (F)  14	  3.1%
Pro (P)  15	  3.3%
Ser (S)  34	  7.5%
Thr (T)  24	  5.3%
Trp (W)   2	  0.4%
Tyr (Y)  17	  3.7%
Val (V)  15	  3.3%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 59
Total number of positively charged residues (Arg + Lys): 53

Atomic composition:

Carbon      C	      2245
Hydrogen    H	      3554
Nitrogen    N	       676
Oxygen      O	       747
Sulfur      S	        12

Formula: C2245H3554N676O747S12
Total number of atoms: 7234

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    36455
Abs 0.1% (=1 g/l)   0.696, assuming all pairs of Cys residues form cystines


Ext. coefficient    36330
Abs 0.1% (=1 g/l)   0.694, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 66.74
This classifies the protein as unstable.



Aliphatic index: 63.39

Grand average of hydropathicity (GRAVY): -1.101