ProtParam
User-provided sequence:
10 20 30 40 50 60
MSKQFVRSAK NMMKGYSSTQ VLVRDATAND SRTPSIDTLD DLAQRSYDSV DFFEIMDMLD
70 80 90 100 110 120
KRLNDKGKYW RHVAKSLTVL DYLVRFGSEN CVLWCRENFY VIKTLREFRH ENESGFDEGQ
130 140 150 160 170 180
IIRVKAKELV SLLNDEERLR EERSMNTRNR RANRAARPRP RRQRTRSNPH DSSPSYQDDL
190 200 210 220 230 240
EKALEESRIT AQEDEQRRRE LAQYDDEDPD FQAALQLSKE EEELKQLQEL QRLQKQQQSL
250 260 270 280 290 300
SQFQAPLQQQ QPQQQPAYYD IFGNPISQDE YLQYQYQQDQ EQAMAQQRWL DQQQEQQQLA
310 320 330 340 350 360
EQQYFQQQQQ AAAAASALQQ QQTAANMQQQ QQQPADFQQP LPTGSNNPFS MDNLERQKQE
370 380 390 400 410 420
QQHAQLQRQQ EEARQQQEQL KLQQLQRQQQ EEAQLHQKRQ EEAQLQQQQA QLLQQQAQFQ
430 440 450 460 470 480
QQQPLKQTRT GNQSISDKYS DLNTLLATGT GIDTFGNTGE ARIPAQHTKT GTFINSQGTG
490 500 510 520 530 540
YKQVTNEPKN NPFLSNQYTG LPSTNIVPTQ TGYGFGNQPQ SPPTNSPQQN PTGISYSQPQ
550 560 570 580 590 600
QQQQPQQQPQ YMQNFQQQQP QYAQNFQQQP QYTQNYQQQP QYIQPHQQQQ QQQQQQQQQQ
610
GYTPDQGVSL IDL
References and
documentation are available.
Number of amino acids: 613
Molecular weight: 71807.36
Theoretical pI: 5.32
Total number of negatively charged residues (Asp + Glu): 71
Total number of positively charged residues (Arg + Lys): 59
Atomic composition:
Carbon C 3076
Hydrogen H 4799
Nitrogen N 943
Oxygen O 1027
Sulfur S 12
Formula: C
3076H
4799N
943O
1027S
12
Total number of atoms: 9857
Extinction coefficients:
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 52385
Abs 0.1% (=1 g/l) 0.730, assuming all pairs of Cys residues form cystines
Ext. coefficient 52260
Abs 0.1% (=1 g/l) 0.728, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 77.32
This classifies the protein as unstable.
Aliphatic index: 52.90
Grand average of hydropathicity (GRAVY): -1.373