ProtParam
User-provided sequence:
10 20 30 40 50 60
MSKQQIGVVG MAVMGRNLAL NIESRGYTVS IFNRSREKTE EVIAENPGKK LVPYYTVKEF
70 80 90 100 110 120
VESLETPRRI LLMVKAGAGT DAAIDSLKPY LDKGDIIIDG GNTFFQDTIR RNRELSAEGF
130 140 150 160 170 180
NFIGTGVSGG EEGALKGPSI MPGGQKEAYE LVAPILTKIA AVAEDGEPCV TYIGADGAGH
190 200 210 220 230 240
YVKMVHNGIE YGDMQLIAEA YSLLKGGLNL TNEELAQTFT EWNNGELSSY LIDITKDIFT
250 260 270 280 290 300
KKDEDGNYLV DVILDEAANK GTGKWTSQSA LDLGEPLSLI TESVFARYIS SLKDQRVAAS
310 320 330 340 350 360
KVLSGPQAQP AGDKAEFIEK VRRALYLGKI VSYAQGFSQL RAASEEYNWD LNYGEIAKIF
370 380 390 400 410 420
RAGCIIRAQF LQKITDAYAE NPQIANLLLA PYFKQIADDY QQALRDVVAY AVQNGIPVPT
430 440 450 460
FSAAVAYYDS YRAAVLPANL IQAQRDYFGA HTYKRIDKEG VFHTEWLD
References and
documentation are available.
Number of amino acids: 468
Molecular weight: 51481.31
Theoretical pI: 5.04
Total number of negatively charged residues (Asp + Glu): 61
Total number of positively charged residues (Arg + Lys): 48
Atomic composition:
Carbon C 2311
Hydrogen H 3613
Nitrogen N 609
Oxygen O 704
Sulfur S 9
Formula: C
2311H
3613N
609O
704S
9
Total number of atoms: 7246
Extinction coefficients:
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 59375
Abs 0.1% (=1 g/l) 1.153, assuming all pairs of Cys residues form cystines
Ext. coefficient 59250
Abs 0.1% (=1 g/l) 1.151, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 35.98
This classifies the protein as stable.
Aliphatic index: 91.37
Grand average of hydropathicity (GRAVY): -0.200