ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MSKQTTSQVG AIADIGWPDM FNSGVMMLIP DADTASVLQN YIFENTSIDG SDQGILNQFF 

        70         80         90        100        110        120 
NQNCCTDELV KDSFSREWVQ LSFTYNVTIP NLGYQSSPAM NYFKPSIKLI HFIGKHKPWS 

       130        140        150        160        170        180 
LWSQKNFIKN EYHDQWNEVY EEFKEEHQLN NEVSKPKISD SDKTETPETI TPVDAPPSNE 

       190        200        210        220        230        240 
PTTNQEIDTI STVEENVDNQ NAEPVPNSDH SPAPNPVPLD FTKWLTTFIN KDHLTNQPVN 

       250        260        270        280        290        300 
ESREYSKEND NNIINSSSNR DQESPPNSTQ ELNSSYSVVS TQADSDEHQN AEEEDSTTDN 

       310        320        330        340        350        360 
ASNSGEESHL DDISTAASSN NNVSNQPDGK NFSNSKENNI SVESSPSNPE QKRSTDNIQK 

       370        380        390        400        410        420 
PSVSTNDLPD DVEPHTSVDD NIQYLEKDKE GYEEFLPDVY ESNAIDNEEE FFDDDARDAT 

       430        440        450        460        470        480 
EGETKTSAVA DKQEDMKLTA EETNQPQQEM PNFKFDWEDS DYLSKVERCF PDDIFEYAVE 

References and documentation are available.

Number of amino acids: 480

Molecular weight: 54333.05

Theoretical pI: 4.13

Amino acid composition: 
Ala (A)  20	  4.2%
Arg (R)   6	  1.2%
Asn (N)  50	 10.4%
Asp (D)  46	  9.6%
Cys (C)   3	  0.6%
Gln (Q)  26	  5.4%
Glu (E)  50	 10.4%
Gly (G)  11	  2.3%
His (H)   9	  1.9%
Ile (I)  24	  5.0%
Leu (L)  19	  4.0%
Lys (K)  26	  5.4%
Met (M)   7	  1.5%
Phe (F)  20	  4.2%
Pro (P)  31	  6.5%
Ser (S)  55	 11.5%
Thr (T)  32	  6.7%
Trp (W)   7	  1.5%
Tyr (Y)  13	  2.7%
Val (V)  25	  5.2%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 96
Total number of positively charged residues (Arg + Lys): 32

Atomic composition:

Carbon      C	      2341
Hydrogen    H	      3529
Nitrogen    N	       625
Oxygen      O	       849
Sulfur      S	        10

Formula: C2341H3529N625O849S10
Total number of atoms: 7354

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    57995
Abs 0.1% (=1 g/l)   1.067, assuming all pairs of Cys residues form cystines


Ext. coefficient    57870
Abs 0.1% (=1 g/l)   1.065, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 44.65
This classifies the protein as unstable.



Aliphatic index: 54.21

Grand average of hydropathicity (GRAVY): -1.052