ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MSKRSIEVNE EQDRVVSAKT ESHSVPAIPA SEEQDAPKND LEEQLSDEFD SDGEIIEIDG 

        70         80         90        100        110        120 
DDEINDEDDL RKKQEEAETL VQKDQSEGNK EKIQELYLPH MSRPLGPDEV LEADPTVYEM 

       130        140        150        160        170        180 
LHNVNMPWPC LTLDVIPDTL GSERRNYPQS ILLTTATQSS RKKENELMVL ALSNLAKTLL 

       190        200        210        220        230        240 
KDDNEGEDDE EDDEDDVDPV IENENIPLRD TTNRLKVSPF AISNQEVLTA TMSENGDVYI 

       250        260        270        280        290        300 
YNLAPQSKAF STPGYQIPKS AKRPIHTVKN HGNVEGYGLD WSPLIKTGAL LSGDCSGQIY 

       310        320        330        340        350        360 
FTQRHTSRWV TDKQPFTVSN NKSIEDIQWS RTESTVFATA GCDGYIRIWD TRSKKHKPAI 

       370        380        390        400        410        420 
SVKASNTDVN VISWSDKIGY LLASGDDNGT WGVWDLRQFT PSNADAVQPV AQYDFHKGAI 

       430        440        450        460        470        480 
TSIAFNPLDE SIVAVGSEDN TVTLWDLSVE ADDEEIKQQA AETKELQEIP PQLLFVHWQK 

       490        500        510 
EVKDVKWHKQ IPGCLVSTGT DGLNVWKTIS V 

References and documentation are available.

Number of amino acids: 511

Molecular weight: 57261.30

Theoretical pI: 4.48

Amino acid composition: 
Ala (A)  29	  5.7%
Arg (R)  16	  3.1%
Asn (N)  27	  5.3%
Asp (D)  48	  9.4%
Cys (C)   4	  0.8%
Gln (Q)  25	  4.9%
Glu (E)  46	  9.0%
Gly (G)  25	  4.9%
His (H)  10	  2.0%
Ile (I)  31	  6.1%
Leu (L)  39	  7.6%
Lys (K)  33	  6.5%
Met (M)   6	  1.2%
Phe (F)  10	  2.0%
Pro (P)  27	  5.3%
Ser (S)  42	  8.2%
Thr (T)  34	  6.7%
Trp (W)  12	  2.3%
Tyr (Y)  11	  2.2%
Val (V)  36	  7.0%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 94
Total number of positively charged residues (Arg + Lys): 49

Atomic composition:

Carbon      C	      2506
Hydrogen    H	      3918
Nitrogen    N	       676
Oxygen      O	       839
Sulfur      S	        10

Formula: C2506H3918N676O839S10
Total number of atoms: 7949

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    82640
Abs 0.1% (=1 g/l)   1.443, assuming all pairs of Cys residues form cystines


Ext. coefficient    82390
Abs 0.1% (=1 g/l)   1.439, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 52.82
This classifies the protein as unstable.



Aliphatic index: 79.53

Grand average of hydropathicity (GRAVY): -0.663