ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MSKSLEWDNL GFSLLPWIRT GLDVMGFETM TPVQASTIPM LAGNKDVVVD SVTGSGKTAA 

        70         80         90        100        110        120 
FVIPVLEKVV KEEANTSKFK KAHFHSLIIA PTRELSRQIE SVVLSFLEHY PSDLFPIKCQ 

       130        140        150        160        170        180 
LLVGTNEATV RDDVSNFLRN RPQILIGTPG RVLDFLQMPA VKTSACSMVV MDEADRLLDM 

       190        200        210        220        230        240 
SFIKDTEKIL RLLPKQRRTG LFSATMRSAG SDIFKTGLRN PVRITVNSKN QAPSSLKLNY 

       250        260        270        280        290        300 
CVVNPAEKLQ LLVSILNNYK FKKCIVYFPT CVSVSYFYSF IQYLGKRNIL VNEVEIFSLH 

       310        320        330        340        350        360 
GKLQTSARTK TLTAFTDSLS NSVLFTTDVA ARGIDIPDVD LVIQLDPPTN TDMFMHRCGR 

       370        380        390        400        410        420 
TGRANRVGKA ITFLNEGREE DFIPFMQVKN VELEELDLEV KGITTNFYED FRNWILEDRD 

       430        440        450        460        470        480 
RFDKGVKAYV AFIKYYSNHS ATSIFRLQSL DYVGIAKLYG LFRLPRMPEI TKYLATEKQE 

       490        500        510        520        530        540 
GIFPGNWLVD PPVNMDEYKY KDKKREKERQ ETLKNISLIN DKKKLKSELK KKNLAWSDKT 

       550        560        570        580        590        600 
LTKERKLERK EKMSLKRKAI EEELKAEELD ENAEEERIKE DWKEIVLQNK RKKVSSKAIQ 


GNFDDL 

References and documentation are available.

Number of amino acids: 606

Molecular weight: 69422.28

Theoretical pI: 9.11

Amino acid composition: 
Ala (A)  31	  5.1%
Arg (R)  35	  5.8%
Asn (N)  31	  5.1%
Asp (D)  36	  5.9%
Cys (C)   6	  1.0%
Gln (Q)  17	  2.8%
Glu (E)  45	  7.4%
Gly (G)  26	  4.3%
His (H)   6	  1.0%
Ile (I)  36	  5.9%
Leu (L)  64	 10.6%
Lys (K)  59	  9.7%
Met (M)  15	  2.5%
Phe (F)  31	  5.1%
Pro (P)  24	  4.0%
Ser (S)  41	  6.8%
Thr (T)  37	  6.1%
Trp (W)   6	  1.0%
Tyr (Y)  16	  2.6%
Val (V)  44	  7.3%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 81
Total number of positively charged residues (Arg + Lys): 94

Atomic composition:

Carbon      C	      3116
Hydrogen    H	      4998
Nitrogen    N	       836
Oxygen      O	       911
Sulfur      S	        21

Formula: C3116H4998N836O911S21
Total number of atoms: 9882

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    57215
Abs 0.1% (=1 g/l)   0.824, assuming all pairs of Cys residues form cystines


Ext. coefficient    56840
Abs 0.1% (=1 g/l)   0.819, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 36.55
This classifies the protein as stable.



Aliphatic index: 90.53

Grand average of hydropathicity (GRAVY): -0.356