ProtParam
User-provided sequence:
10 20 30 40 50 60
MSKSQIESTW SSLLQELEII WKEVGETETE REKILIEIEE ECREVYNRKI EKVKEEKIRI
70 80 90 100 110 120
KQEIADSEAR VIDICSVMEE PPILGRHHQS DQQSGNGRSL KDELVKILQK LEEMEKRKSE
130 140 150 160 170 180
RKIQFIQVID DIRCVREEIN GESDDETCSS DFSADESDLS LRKLEELHRE LYTLQEQKRN
190 200 210 220 230 240
RVKQIQDNIR TLESLCSVLG LNFRETVTKI HPSLVDTEGS RSISNETLDK LASSVQQWHE
250 260 270 280 290 300
TKIQRMQELQ DLVTTMLEFW NLMDTPAEEQ QKFMDVSCNI AATVSEITKP NSLSIDLLEE
310 320 330 340 350 360
VKAELCRLEE LKWSKMKELV LKKRSELEEI CRRTHIVLEE EDIAVENVIK AIESGDVNPE
370 380 390 400 410 420
NILEQIEYRA GKVKEEALSR KEILEKADKW LNACEEENWL EEYNQDENRY NAGKGSHLIL
430 440 450 460 470 480
KRAEKARALV NKLPAMVEAL ASKITIWESE KEYEFLFDGN RLLSMLEEYT ELREEKEQER
490 500 510 520 530 540
RRKRDLKKHQ GQVTSEQDKG SVTKPQSAKK GLKVSTNKRF VSSPHTPQTD SPHSAKSNQS
FSTPLSRHG
References and
documentation are available.
Number of amino acids: 549
Molecular weight: 63869.11
Theoretical pI: 5.20
Total number of negatively charged residues (Asp + Glu): 111
Total number of positively charged residues (Arg + Lys): 87
Atomic composition:
Carbon C 2764
Hydrogen H 4511
Nitrogen N 789
Oxygen O 904
Sulfur S 19
Formula: C
2764H
4511N
789O
904S
19
Total number of atoms: 8987
Extinction coefficients:
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 54930
Abs 0.1% (=1 g/l) 0.860, assuming all pairs of Cys residues form cystines
Ext. coefficient 54430
Abs 0.1% (=1 g/l) 0.852, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 64.91
This classifies the protein as unstable.
Aliphatic index: 84.86
Grand average of hydropathicity (GRAVY): -0.830