ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MSKTAVKDSA TEKTKLSESE QHYFNSYDHY GIHEEMLQDT VRTLSYRNAI IQNKDLFKDK 

        70         80         90        100        110        120 
IVLDVGCGTG ILSMFAAKHG AKHVIGVDMS SIIEMAKELV ELNGFSDKIT LLRGKLEDVH 

       130        140        150        160        170        180 
LPFPKVDIII SEWMGYFLLY ESMMDTVLYA RDHYLVEGGL IFPDKCSIHL AGLEDSQYKD 

       190        200        210        220        230        240 
EKLNYWQDVY GFDYSPFVPL VLHEPIVDTV ERNNVNTTSD KLIEFDLNTV KISDLAFKSN 

       250        260        270        280        290        300 
FKLTAKRQDM INGIVTWFDI VFPAPKGKRP VEFSTGPHAP YTHWKQTIFY FPDDLDAETG 

       310        320        330        340 
DTIEGELVCS PNEKNNRDLN IKISYKFESN GIDGNSRSRK NEGSYLMH 

References and documentation are available.

Number of amino acids: 348

Molecular weight: 39786.12

Theoretical pI: 5.26

Amino acid composition: 
Ala (A)  14	  4.0%
Arg (R)  10	  2.9%
Asn (N)  18	  5.2%
Asp (D)  29	  8.3%
Cys (C)   3	  0.9%
Gln (Q)   7	  2.0%
Glu (E)  24	  6.9%
Gly (G)  21	  6.0%
His (H)  12	  3.4%
Ile (I)  25	  7.2%
Leu (L)  30	  8.6%
Lys (K)  28	  8.0%
Met (M)  10	  2.9%
Phe (F)  18	  5.2%
Pro (P)  13	  3.7%
Ser (S)  25	  7.2%
Thr (T)  19	  5.5%
Trp (W)   4	  1.1%
Tyr (Y)  16	  4.6%
Val (V)  22	  6.3%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 53
Total number of positively charged residues (Arg + Lys): 38

Atomic composition:

Carbon      C	      1792
Hydrogen    H	      2755
Nitrogen    N	       459
Oxygen      O	       540
Sulfur      S	        13

Formula: C1792H2755N459O540S13
Total number of atoms: 5559

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    45965
Abs 0.1% (=1 g/l)   1.155, assuming all pairs of Cys residues form cystines


Ext. coefficient    45840
Abs 0.1% (=1 g/l)   1.152, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 38.34
This classifies the protein as stable.



Aliphatic index: 83.99

Grand average of hydropathicity (GRAVY): -0.378