ProtParam
User-provided sequence:
10 20 30 40 50 60
MSKTLVQPVG QKRLTNVAVV RLKKQGNRFE IACYKNKVLS WRSGVEKDID EVLQSHTVYS
70 80 90 100 110 120
NVSKGVLAKS KDLMKSFGSD DHTKICIDIL EKGELQVAGK ERESQFSSQF RDIATIVMQK
130 140 150 160 170 180
TINPETQRPY TISMVERLMH EIHFAVDPHS NSKKQALDVI RELQKHFPIK RSPMRLRLTV
190 200 210 220 230 240
PVQNFPSLLE KLKEWDGSVV SKDESGTQMS TVCEMEPGLF RECDSHVRSI QGRLEILAVS
250 260 270 280 290 300
VHAEGDTSMD HYDEHDDMAL QTHKPLLPAE TETKDLTDPV VELSKKLQKQ EISTTDNIKQ
310 320 330 340 350 360
EGGEEKKGTK CSTCNTFVGE AKQYREHCKS DWHKHNLKRK TRKLPPISAD ECMSEIDMDD
370
SRADLKDYSF
References and
documentation are available.
Number of amino acids: 370
Molecular weight: 42231.08
Theoretical pI: 6.93
Total number of negatively charged residues (Asp + Glu): 58
Total number of positively charged residues (Arg + Lys): 57
Atomic composition:
Carbon C 1833
Hydrogen H 2965
Nitrogen N 525
Oxygen O 577
Sulfur S 20
Formula: C
1833H
2965N
525O
577S
20
Total number of atoms: 5920
Extinction coefficients:
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 25940
Abs 0.1% (=1 g/l) 0.614, assuming all pairs of Cys residues form cystines
Ext. coefficient 25440
Abs 0.1% (=1 g/l) 0.602, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 50.30
This classifies the protein as unstable.
Aliphatic index: 74.49
Grand average of hydropathicity (GRAVY): -0.712