ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MSKTPSFDKD RDYIGFATLP EQVHRKSVKR GFEFTLMVVG ESGLGKSTLI NSLFLGDLYK 

        70         80         90        100        110        120 
NRQMPNVEER IEKTTKVEKK TMDIEERGVR LRLTVVDTPG FGDAINCEDS WRVCTQYIDE 

       130        140        150        160        170        180 
QFRQYFTDES GLNRRNIQDN RVHCCLYFVP PWGHSLRQMD LDLIRRLHRK VNIVLVIGKA 

       190        200        210        220        230        240 
DCLNKQEVRK LKERILQDLE DNHIQLYQFP ECDSDEDDDF KQQDRELKAS IPFAVVGSNT 

       250        260        270        280        290        300 
ILEVAGKKVR GRQYPWGVVN VEDPEHSDFI KLRTFLISTH MQDLKDTTQD VHYENFRAQC 

       310        320        330        340        350        360 
ISQISQHALR ERGKLKRDSI SSTNGFDAAI SETDRLLLQK DEEIRRMQDM LTQMQEKLKQ 

       370 
THLMEMKKND SVIDV 

References and documentation are available.

Number of amino acids: 375

Molecular weight: 43836.84

Theoretical pI: 6.25

Amino acid composition: 
Ala (A)  10	  2.7%
Arg (R)  30	  8.0%
Asn (N)  15	  4.0%
Asp (D)  34	  9.1%
Cys (C)   7	  1.9%
Gln (Q)  24	  6.4%
Glu (E)  28	  7.5%
Gly (G)  18	  4.8%
His (H)  10	  2.7%
Ile (I)  22	  5.9%
Leu (L)  34	  9.1%
Lys (K)  28	  7.5%
Met (M)  11	  2.9%
Phe (F)  16	  4.3%
Pro (P)  10	  2.7%
Ser (S)  21	  5.6%
Thr (T)  20	  5.3%
Trp (W)   3	  0.8%
Tyr (Y)   8	  2.1%
Val (V)  26	  6.9%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 62
Total number of positively charged residues (Arg + Lys): 58

Atomic composition:

Carbon      C	      1914
Hydrogen    H	      3065
Nitrogen    N	       555
Oxygen      O	       588
Sulfur      S	        18

Formula: C1914H3065N555O588S18
Total number of atoms: 6140

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    28795
Abs 0.1% (=1 g/l)   0.657, assuming all pairs of Cys residues form cystines


Ext. coefficient    28420
Abs 0.1% (=1 g/l)   0.648, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 46.65
This classifies the protein as unstable.



Aliphatic index: 81.01

Grand average of hydropathicity (GRAVY): -0.689