ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MSKTRYYLEQ CIPEMDDLVE KGLFTKNEVS LIMKKRTDFE HRLNSRGSSI NDYIKYINYE 

        70         80         90        100        110        120 
SNVNKLRAKR CKRILQVKKT NSLSDWSIQQ RIGFIYQRGT NKFPQDLKFW AMYLNYMKAR 

       130        140        150        160        170        180 
GNQTSYKKIH NIYNQLLKLH PTNVDIWISC AKYEYEVHAN FKSCRNIFQN GLRFNPDVPK 

       190        200        210        220        230        240 
LWYEYVKFEL NFITKLINRR KVMGLINERE QELDMQNEQK NNQAPDEEKS HLQVPSTGDS 

       250        260        270        280        290        300 
MKDKLNELPE ADISVLGNAE TNPALRGDIA LTIFDVCMKT LGKHYINKHK GYYAISDSKM 

       310        320        330        340        350        360 
NIELNKETLN YLFSESLRYI KLFDEFLDLE RDYLINHVLQ FWKNDMYDLS LRKDLPELYL 

       370        380        390        400        410        420 
KTVMIDITLN IRYMPVEKLD IDQLQLSVKK YFAYISKLDS ASVKSLKNEY RSYLQDNYLK 

       430        440 
KMNAEDDPRY KILDLIISKL 

References and documentation are available.

Number of amino acids: 440

Molecular weight: 52418.36

Theoretical pI: 9.03

Amino acid composition: 
Ala (A)  14	  3.2%
Arg (R)  22	  5.0%
Asn (N)  36	  8.2%
Asp (D)  30	  6.8%
Cys (C)   5	  1.1%
Gln (Q)  18	  4.1%
Glu (E)  28	  6.4%
Gly (G)  12	  2.7%
His (H)   8	  1.8%
Ile (I)  33	  7.5%
Leu (L)  51	 11.6%
Lys (K)  47	 10.7%
Met (M)  14	  3.2%
Phe (F)  16	  3.6%
Pro (P)  12	  2.7%
Ser (S)  28	  6.4%
Thr (T)  15	  3.4%
Trp (W)   5	  1.1%
Tyr (Y)  29	  6.6%
Val (V)  17	  3.9%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 58
Total number of positively charged residues (Arg + Lys): 69

Atomic composition:

Carbon      C	      2360
Hydrogen    H	      3710
Nitrogen    N	       628
Oxygen      O	       683
Sulfur      S	        19

Formula: C2360H3710N628O683S19
Total number of atoms: 7400

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    70960
Abs 0.1% (=1 g/l)   1.354, assuming all pairs of Cys residues form cystines


Ext. coefficient    70710
Abs 0.1% (=1 g/l)   1.349, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 41.58
This classifies the protein as unstable.



Aliphatic index: 88.84

Grand average of hydropathicity (GRAVY): -0.628