ProtParam
User-provided sequence:
10 20 30 40 50 60
MSLASVAVAA PSFTTGTVHG DAAPILASVN TEAIPNSYIV KFKNHVNEKD VSTHHSWVQS
70 80 90 100 110 120
LHDQGEEKRL ELRRRGMDFG ILSGVKHTFK IGETFKGYAG QFDDELIEQL RRHPDVEYIE
130 140 150 160 170 180
KDTVVHTMQP VSSEETCSSE VEKNSPWGLA RISHRDTLSF GTFNKYLYSS EGGEGVDVYV
190 200 210 220 230 240
IDTGCNVDHV DFEGRATWGK TIPSGVSDLD GNGHGTHCSG TIAGKKYGVA KKARVYAVKV
250 260 270 280 290 300
LRSNGSGTMS DVVKGVEWAA NAHLEALKQA KAGKRKGFKG SAANMSLGGG KTVALEEVVN
310 320 330 340 350 360
AAVEAGVHFA VAAGNDNADA CNYSPAAAQN AVTVGASALD DSRAYFSNWG KCTDIFAPGL
370 380 390 400 410 420
SIQSTWIGSK HAVNTISGTS MASPHIAGLL AYYLSLQPAA DSEYAVAAIT PAELKANIIS
430 440 450 460 470 480
IATEGALSDL PKDTPNVLAW NGGGCSNYSS ILEQGSYKVK SSKNSKLDIE ALEKAIENDL
490 500
NIVSGQIVKG AESLASKAQK FST
References and
documentation are available.
Number of amino acids: 503
Molecular weight: 53280.56
Theoretical pI: 6.07
Total number of negatively charged residues (Asp + Glu): 56
Total number of positively charged residues (Arg + Lys): 48
Atomic composition:
Carbon C 2339
Hydrogen H 3675
Nitrogen N 653
Oxygen O 747
Sulfur S 12
Formula: C
2339H
3675N
653O
747S
12
Total number of atoms: 7426
Extinction coefficients:
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 61225
Abs 0.1% (=1 g/l) 1.149, assuming all pairs of Cys residues form cystines
Ext. coefficient 60850
Abs 0.1% (=1 g/l) 1.142, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 35.25
This classifies the protein as stable.
Aliphatic index: 79.56
Grand average of hydropathicity (GRAVY): -0.241