ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MSLAVLLNKE DKDISDFSKT TAGKSAKKNS RERVADVAPT RVLDKKQAYL SQLNSEFNRI 

        70         80         90        100        110        120 
KRRDSIEQLY QDWKFINLQE FELISEWNQQ SKDWQFDNTN DSQDLHFKKL YRDMSMINKE 

       130        140        150        160        170        180 
WAEYQSFKNA NLSDIINEKD ADEDEEDDED ELEDGEEDME EDEASTGRHT NGKSMRGNGI 

       190        200        210        220        230        240 
QKSRKKDAAA AAAIGKAIKD DQTHADTVVT VNGDENEDGN NGEDEDNDND NENNNDNDND 

       250        260        270        280        290        300 
NENENDNDSD NDDEEENGEE DEEEEEIEDL DEEDFAAFEE QDDNDDEDFN PDVEKRRKRS 

       310        320        330        340        350        360 
SSSSSSTKLS MNSLSLITSK KINKNITINS DRPKIVRELI KMCNKNKHQK IKKRRFTNCI 

       370        380        390        400        410        420 
VTDYNPIDSK LNIKITLKQY HVKRLKKLIN DAKREREREE ALKNNVGLDG NDLDNDEDGS 

       430        440        450        460        470        480 
ESHKRRKLNN NTANGADDAN KRKFNTRHGL PTYGMKMNAK EARAIQRHYD NTYTTIWKDM 

       490        500        510        520        530        540 
ARKDSTKMSR LVQQIQSIRS TNFRKTSSLC AREAKKWQSK NFKQIKDFQT RARRGIREMS 

       550        560        570        580        590        600 
NFWKKNEREE RDLKKKIEKE AMEQAKKEEE EKESKRQAKK LNFLLTQTEL YSHFIGRKIK 

       610        620        630        640        650        660 
TNELEGNNVS SNDSESQKNI DISALAPNKN DFHAIDFDNE NDEQLRLRAA ENASNALAET 

       670        680        690        700        710        720 
RAKAKQFDDH ANAHEEEEEE DELNFQNPTS LGEITIEQPK ILACTLKEYQ LKGLNWLANL 

       730        740        750        760        770        780 
YDQGINGILA DEMGLGKTVQ SISVLAHLAE NHNIWGPFLV VTPASTLHNW VNEISKFLPQ 

       790        800        810        820        830        840 
FKILPYWGNA NDRKVLRKFW DRKNLRYNKN APFHVMVTSY QMVVTDANYL QKMKWQYMIL 

       850        860        870        880        890        900 
DEAQAIKSSQ SSRWKNLLSF HCRNRLLLTG TPIQNSMQEL WALLHFIMPS LFDSHDEFNE 

       910        920        930        940        950        960 
WFSKDIESHA EANTKLNQQQ LRRLHMILKP FMLRRVKKNV QSELGDKIEI DVLCDLTQRQ 

       970        980        990       1000       1010       1020 
AKLYQVLKSQ ISTNYDAIEN AATNDSTSNS ASNSGSDQNL INAVMQFRKV CNHPDLFERA 

      1030       1040       1050       1060       1070       1080 
DVDSPFSFTT FGKTTSMLTA SVANNNSSVI SNSNMNLSSM SSNNISNGKF TDLIYSSRNP 

      1090       1100       1110       1120       1130       1140 
IKYSLPRLIY EDLILPNYNN DVDIANKLKN VKFNIFNPST NYELCLFLSK LTGEPSLNEF 

      1150       1160       1170       1180       1190       1200 
FRVSTTPLLK RVIERTNGPK NTDSLSFKTI TQELLEVTRN APSEGVMASL LNVEKHAYER 

      1210       1220       1230       1240       1250       1260 
EYLNCIQRGY HPNVSAPPVT IEVLGSSHVT NSINNELFDP LISQALSDIP AITQYNMHVK 

      1270       1280       1290       1300       1310       1320 
KGIPVEDFPK TGLFPEPLNK NFSSNISMPS MDRFITESAK LRKLDELLVK LKSEGHRVLI 

      1330       1340       1350       1360       1370       1380 
YFQMTKMMDL MEEYLTYRQY NHIRLDGSSK LEDRRDLVHD WQTNPEIFVF LLSTRAGGLG 

      1390       1400       1410       1420       1430       1440 
INLTAADTVI FYDSDWNPTI DSQAMDRAHR LGQTRQVTVY RLLVRGTIEE RMRDRAKQKE 

      1450       1460       1470       1480 
QVQQVVMEGK TQEKNIKTIE VGENDSEVTR EGSKSISQDG IKEAASALA 

References and documentation are available.

Number of amino acids: 1489

Molecular weight: 171455.15

Theoretical pI: 5.52

Amino acid composition: 
Ala (A)  88	  5.9%
Arg (R)  82	  5.5%
Asn (N) 141	  9.5%
Asp (D) 116	  7.8%
Cys (C)   9	  0.6%
Gln (Q)  67	  4.5%
Glu (E) 129	  8.7%
Gly (G)  51	  3.4%
His (H)  30	  2.0%
Ile (I)  86	  5.8%
Leu (L) 126	  8.5%
Lys (K) 127	  8.5%
Met (M)  37	  2.5%
Phe (F)  54	  3.6%
Pro (P)  38	  2.6%
Ser (S) 120	  8.1%
Thr (T)  77	  5.2%
Trp (W)  18	  1.2%
Tyr (Y)  34	  2.3%
Val (V)  59	  4.0%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 245
Total number of positively charged residues (Arg + Lys): 209

Atomic composition:

Carbon      C	      7435
Hydrogen    H	     11796
Nitrogen    N	      2148
Oxygen      O	      2419
Sulfur      S	        46

Formula: C7435H11796N2148O2419S46
Total number of atoms: 23844

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient   150160
Abs 0.1% (=1 g/l)   0.876, assuming all pairs of Cys residues form cystines


Ext. coefficient   149660
Abs 0.1% (=1 g/l)   0.873, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 45.59
This classifies the protein as unstable.



Aliphatic index: 72.93

Grand average of hydropathicity (GRAVY): -0.887