ProtParam
User-provided sequence:
10 20 30 40 50 60
MSLEATDSKA MVLLMGVRRC GKSSICKVVF HNMQPLDTLY LESTSNPSLE HFSTLIDLAV
70 80 90 100 110 120
MELPGQLNYF EPSYDSERLF KSVGALVYVI DSQDEYINAI TNLAMIIEYA YKVNPSINIE
130 140 150 160 170 180
VLIHKVDGLS EDFKVDAQRD IMQRTGEELL ELGLDGVQVS FYLTSIFDHS IYEAFSRIVQ
190 200 210 220 230 240
KLIPELSFLE NMLDNLIQHS KIEKAFLFDV NSKIYVSTDS NPVDIQMYEV CSEFIDVTID
250 260 270 280 290 300
LFDLYKAPVL RNSQKSSDKD NVINPRNELQ NVSQLANGVI IYLRQMIRGL ALVAIIRPNG
310 320 330 340
TDMESCLTVA DYNIDIFKKG LEDIWANARA SQAKNSIEDD V
References and
documentation are available.
Number of amino acids: 341
Molecular weight: 38600.10
Theoretical pI: 4.57
Total number of negatively charged residues (Asp + Glu): 50
Total number of positively charged residues (Arg + Lys): 29
Atomic composition:
Carbon C 1722
Hydrogen H 2724
Nitrogen N 440
Oxygen O 533
Sulfur S 15
Formula: C
1722H
2724N
440O
533S
15
Total number of atoms: 5434
Extinction coefficients:
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 26610
Abs 0.1% (=1 g/l) 0.689, assuming all pairs of Cys residues form cystines
Ext. coefficient 26360
Abs 0.1% (=1 g/l) 0.683, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 37.58
This classifies the protein as stable.
Aliphatic index: 106.30
Grand average of hydropathicity (GRAVY): -0.025