ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MSLEEFDEVK YDHSTKRLDT PSRYLLRKAR RNPNGLQELR ESMKSSTIYV GNLSFYTSEE 

        70         80         90        100        110        120 
QIYELFSKCG TIKRIIMGLD RFKFTPCGFC FIIYSCPDEA LNALKYLSDT KLDEKTITID 

       130        140        150        160        170        180 
LDPGFEDGRQ FGRGKSGGQV SDELRFDFDA SRGGFAIPFA ERVGVPHSRF DNSSSQSNTN 

       190        200 
NYIPPPDAMG TFRPGFDEER EDDNYVPQ 

References and documentation are available.

Number of amino acids: 208

Molecular weight: 23774.47

Theoretical pI: 5.02

Amino acid composition: 
Ala (A)   7	  3.4%
Arg (R)  16	  7.7%
Asn (N)   9	  4.3%
Asp (D)  18	  8.7%
Cys (C)   4	  1.9%
Gln (Q)   6	  2.9%
Glu (E)  16	  7.7%
Gly (G)  16	  7.7%
His (H)   2	  1.0%
Ile (I)  11	  5.3%
Leu (L)  15	  7.2%
Lys (K)  11	  5.3%
Met (M)   4	  1.9%
Phe (F)  16	  7.7%
Pro (P)  12	  5.8%
Ser (S)  19	  9.1%
Thr (T)  11	  5.3%
Trp (W)   0	  0.0%
Tyr (Y)   9	  4.3%
Val (V)   6	  2.9%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 34
Total number of positively charged residues (Arg + Lys): 27

Atomic composition:

Carbon      C	      1049
Hydrogen    H	      1604
Nitrogen    N	       286
Oxygen      O	       331
Sulfur      S	         8

Formula: C1049H1604N286O331S8
Total number of atoms: 3278

Extinction coefficients:

This protein does not contain any Trp residues. Experience shows that
this could result in more than 10% error in the computed extinction coefficient.

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    13660
Abs 0.1% (=1 g/l)   0.575, assuming all pairs of Cys residues form cystines


Ext. coefficient    13410
Abs 0.1% (=1 g/l)   0.564, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 47.29
This classifies the protein as unstable.



Aliphatic index: 60.48

Grand average of hydropathicity (GRAVY): -0.703