ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MSLEREEPQH FGAGPAQMPT PVLQQAAKDL INFNDIGLGI GEISHRSKDA TKVIEDSKKH 

        70         80         90        100        110        120 
LIELLNIPDT HEVFYLQGGG TTGFSSVATN LAAAYVGKHG KIAPAGYLVT GSWSQKSFEE 

       130        140        150        160        170        180 
AKRLHVPAEV IFNAKDYNNG KFGKIPDESL WEDKIKGKAF SYVYLCENET VHGVEWPELP 

       190        200        210        220        230        240 
KCLVNDPNIE IVADLSSDIL SRKIDVSQYG VIMAGAQKNI GLAGLTLYII KKSILKNISG 

       250        260        270        280        290        300 
ASDETLHELG VPITPIAFDY PTVVKNNSAY NTIPIFTLHV MDLVFQHILK KGGVEAQQAE 

       310        320        330        340        350        360 
NEEKAKILYE ALDANSDFYN VPVDPKCRSK MNVVFTLKKD GLDDQFLKEA AARHLTGLKG 

       370        380        390 
HRSVGGFRAS IYNALSVKAV QNLVDFIKEF AEKNA 

References and documentation are available.

Number of amino acids: 395

Molecular weight: 43415.57

Theoretical pI: 6.08

Amino acid composition: 
Ala (A)  35	  8.9%
Arg (R)   8	  2.0%
Asn (N)  22	  5.6%
Asp (D)  22	  5.6%
Cys (C)   3	  0.8%
Gln (Q)  13	  3.3%
Glu (E)  27	  6.8%
Gly (G)  30	  7.6%
His (H)  12	  3.0%
Ile (I)  29	  7.3%
Leu (L)  35	  8.9%
Lys (K)  35	  8.9%
Met (M)   5	  1.3%
Phe (F)  17	  4.3%
Pro (P)  17	  4.3%
Ser (S)  24	  6.1%
Thr (T)  16	  4.1%
Trp (W)   3	  0.8%
Tyr (Y)  13	  3.3%
Val (V)  29	  7.3%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 49
Total number of positively charged residues (Arg + Lys): 43

Atomic composition:

Carbon      C	      1958
Hydrogen    H	      3078
Nitrogen    N	       516
Oxygen      O	       582
Sulfur      S	         8

Formula: C1958H3078N516O582S8
Total number of atoms: 6142

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    35995
Abs 0.1% (=1 g/l)   0.829, assuming all pairs of Cys residues form cystines


Ext. coefficient    35870
Abs 0.1% (=1 g/l)   0.826, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 36.29
This classifies the protein as stable.



Aliphatic index: 93.34

Grand average of hydropathicity (GRAVY): -0.206