ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MSLFFKPVIS PQWSFPVLLK IGVRSYAGGP RTKHKGNSPL ASVPTGSSNK NRKQKAKGKK 

        70         80         90        100        110        120 
GNKKNDPDQA FNFGEYGGLK KDVEMNMDST NKLIQKISNF DQLLILPPVR DAVKEIISKE 

       130        140        150        160        170        180 
SLKLQDSRKK TSENIIPSPI QTVAIKRISK NLMDPKLQIH AIAAETGSGK TMAYLIPLID 

       190        200        210        220        230        240 
YLKRQELETP ELWETLRKNV LIRSIILVPT HELVDQVYET VSKTKTLLGL NSFKWDKATS 

       250        260        270        280        290        300 
YRDLLENIKN RIDILVTTPG KLLNLFSIRM ITRPDKVLSK VGFVVLDEAD TLLDRSWLEE 

       310        320        330        340        350        360 
THSAIKRIPN INHLIFCSAT IPQEFNKTMQ RLFPTVVPIM TPRLHKLPFA LDFKVINSAL 

       370        380        390        400        410        420 
SPFKGSKIKA LAQTLYAISN DDTEPGFEKR CIIFVNEKKN VPEIVNLLNK KFGHNAIGLT 

       430        440        450        460        470        480 
GEDTFEERSE KIMPFLSPPR PLSEVVAQST SPPTSLKKFE IPDSNIVIGK LKNTNSNGTA 

       490        500        510        520        530        540 
PSNKSLHVLV TTDLMARGLN FKGVRNVVLY DVPKTSIDLI HRVGRTARMK QGGRVFMLTD 

       550        560 
SKTKSWAKAL PKIIKKHQRL S 

References and documentation are available.

Number of amino acids: 561

Molecular weight: 63057.71

Theoretical pI: 9.95

Amino acid composition: 
Ala (A)  26	  4.6%
Arg (R)  27	  4.8%
Asn (N)  33	  5.9%
Asp (D)  26	  4.6%
Cys (C)   2	  0.4%
Gln (Q)  16	  2.9%
Glu (E)  27	  4.8%
Gly (G)  27	  4.8%
His (H)  10	  1.8%
Ile (I)  45	  8.0%
Leu (L)  60	 10.7%
Lys (K)  62	 11.1%
Met (M)  12	  2.1%
Phe (F)  23	  4.1%
Pro (P)  36	  6.4%
Ser (S)  44	  7.8%
Thr (T)  37	  6.6%
Trp (W)   5	  0.9%
Tyr (Y)   8	  1.4%
Val (V)  35	  6.2%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 53
Total number of positively charged residues (Arg + Lys): 89

Atomic composition:

Carbon      C	      2842
Hydrogen    H	      4644
Nitrogen    N	       778
Oxygen      O	       806
Sulfur      S	        14

Formula: C2842H4644N778O806S14
Total number of atoms: 9084

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    39545
Abs 0.1% (=1 g/l)   0.627, assuming all pairs of Cys residues form cystines


Ext. coefficient    39420
Abs 0.1% (=1 g/l)   0.625, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 39.65
This classifies the protein as stable.



Aliphatic index: 95.72

Grand average of hydropathicity (GRAVY): -0.321