ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MSLFLKDDSI QIREVWNDNL ESEMALIREV VDDFPFVAMD TEFPGIVCRP VGTFKTNTEY 

        70         80         90        100        110        120 
HYETLKTNVN ILKMIQLGLT FSDEKGNLPT CGTDNKYCIW QFNFREFDLE SDIYATDSIE 

       130        140        150        160        170        180 
LLRQSGIDFV KNNEFGIDSK RFAELLMSSG IVLNENVHWV TFHSGYDFGY LLKLLTCQNL 

       190        200        210        220        230        240 
PETQTGFFEM ISVYFPRVYD IKHLMKFCNS LHGGLNKLAE LLDVERVGIC HQAGSDSLLT 

       250        260        270 
SCTFRKLQEN FFIGSMEKYS GVLYGLGVEN GQIVH 

References and documentation are available.

Number of amino acids: 275

Molecular weight: 31542.96

Theoretical pI: 4.84

Amino acid composition: 
Ala (A)   6	  2.2%
Arg (R)   9	  3.3%
Asn (N)  17	  6.2%
Asp (D)  17	  6.2%
Cys (C)   7	  2.5%
Gln (Q)   9	  3.3%
Glu (E)  21	  7.6%
Gly (G)  20	  7.3%
His (H)   7	  2.5%
Ile (I)  17	  6.2%
Leu (L)  31	 11.3%
Lys (K)  14	  5.1%
Met (M)   8	  2.9%
Phe (F)  21	  7.6%
Pro (P)   6	  2.2%
Ser (S)  18	  6.5%
Thr (T)  16	  5.8%
Trp (W)   3	  1.1%
Tyr (Y)  10	  3.6%
Val (V)  18	  6.5%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 38
Total number of positively charged residues (Arg + Lys): 23

Atomic composition:

Carbon      C	      1423
Hydrogen    H	      2173
Nitrogen    N	       359
Oxygen      O	       422
Sulfur      S	        15

Formula: C1423H2173N359O422S15
Total number of atoms: 4392

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    31775
Abs 0.1% (=1 g/l)   1.007, assuming all pairs of Cys residues form cystines


Ext. coefficient    31400
Abs 0.1% (=1 g/l)   0.995, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 34.39
This classifies the protein as stable.



Aliphatic index: 89.24

Grand average of hydropathicity (GRAVY): -0.103