ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MSLHLFLRVL IFQTSPIFKF QLSIVAVKLI ILFMKLMPPI DNNNDEIPCS KTYSAEEIDQ 

        70         80         90        100        110        120 
FATRSFFKSV NNFMRILVLS EPRKGFIRFL IKEIKKTENT NTSLLFMIND DAELEMKPSC 

       130        140        150        160        170        180 
QIYLYSTHDT NHCIMNKLRR WIMLGVQGAL LSNILAPIHI SKIIIGTTPP VPIHDIRNIL 

       190        200        210        220        230        240 
FSKLDIRDRV IPTMEAVKRI SPTKSLDRTF LWFQGLNGLD YSRAKKKAGH YQHLKKILYK 

       250        260 
KWESENNGKW IKRKSNQVDK FWIEVI 

References and documentation are available.

Number of amino acids: 266

Molecular weight: 31143.81

Theoretical pI: 9.80

Amino acid composition: 
Ala (A)   9	  3.4%
Arg (R)  14	  5.3%
Asn (N)  16	  6.0%
Asp (D)  12	  4.5%
Cys (C)   3	  1.1%
Gln (Q)   8	  3.0%
Glu (E)  12	  4.5%
Gly (G)   8	  3.0%
His (H)   7	  2.6%
Ile (I)  32	 12.0%
Leu (L)  28	 10.5%
Lys (K)  26	  9.8%
Met (M)   9	  3.4%
Phe (F)  16	  6.0%
Pro (P)  12	  4.5%
Ser (S)  19	  7.1%
Thr (T)  13	  4.9%
Trp (W)   5	  1.9%
Tyr (Y)   6	  2.3%
Val (V)  11	  4.1%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 24
Total number of positively charged residues (Arg + Lys): 40

Atomic composition:

Carbon      C	      1428
Hydrogen    H	      2277
Nitrogen    N	       377
Oxygen      O	       377
Sulfur      S	        12

Formula: C1428H2277N377O377S12
Total number of atoms: 4471

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    36565
Abs 0.1% (=1 g/l)   1.174, assuming all pairs of Cys residues form cystines


Ext. coefficient    36440
Abs 0.1% (=1 g/l)   1.170, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 41.16
This classifies the protein as unstable.



Aliphatic index: 103.35

Grand average of hydropathicity (GRAVY): -0.119