ProtParam
User-provided sequence:
10 20 30 40 50 60
MSLISKSLAR IECSPFFYPR ASEITTGKRI TSPQIRLYTA AAVGGKTGNG ERKQRAKAPA
70 80 90 100 110 120
KTPEAVTPVK PLEIASVTAT TENELPRPNE IAYESEVANW VNLIGFVDQP VQFEASSDGK
130 140 150 160 170 180
FWAGTVISQR SASDSSGFWI PIIFEGDLAK TAARYVSKDD QIHVSGKLFI DSPPPNMTYA
190 200 210 220 230 240
QANVQVLVQN LNFIQPMSPS PSPFMVMSSS EKEESGIKKQ PARAKQDIVI DEASDSWNHL
250 260 270 280 290 300
IENPKEWWDH RENKVNGLVK PRHPDFKSKD SSFSLWLNKA PNWVLPKLEG LEFDVLVPKA
310 320 330 340 350 360
RVVKQLKGEE SWKDLVQNPD KWWDNRIDKR NAKAPDFKHK ETGEALWLNE SPTWVLPKLP
370
PVKKKQESIV F
References and
documentation are available.
Number of amino acids: 371
Molecular weight: 41729.49
Theoretical pI: 8.84
Total number of negatively charged residues (Asp + Glu): 46
Total number of positively charged residues (Arg + Lys): 50
Atomic composition:
Carbon C 1884
Hydrogen H 2938
Nitrogen N 508
Oxygen O 552
Sulfur S 6
Formula: C
1884H
2938N
508O
552S
6
Total number of atoms: 5888
Extinction coefficients:
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 78950
Abs 0.1% (=1 g/l) 1.892, assuming all pairs of Cys residues form cystines
Ext. coefficient 78950
Abs 0.1% (=1 g/l) 1.892, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 52.09
This classifies the protein as unstable.
Aliphatic index: 76.23
Grand average of hydropathicity (GRAVY): -0.557