ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MSLKNDASSS HDGGSNRESV IDDHGRRKST CGYCKSPARS SISHGLSAQT LTVYDYQALI 

        70         80         90        100        110        120 
DRGWRRSGTY LYKHEMDKTC CPPYTIRLKA SDFVPTKEQQ RVSRRLERFL DGKLDVQPRE 

       130        140        150        160        170        180 
QRGASSSGDV SDTRRKTLGA AKSEENKKVE AVMDDLSKNI DQAVQLCIRS GEFPSNMQIP 

       190        200        210        220        230        240 
KASVKKVFCA RRKKLAEGTE QILYTSNIAF PIAAAIKRIQ TSEKEGINSA EGNRLSPETI 

       250        260        270        280        290        300 
SEMLLSAMHK VGETPDVSIK VCKGHINFLS SAKDSFSDRD VVPNGNISRG ANSLDGSETL 

       310        320        330        340        350        360 
HAKKDSENHQ ARKRKLEIHL KRSSFDPEEH ELYKRYQLKV HNDKPGHVVE SSYRRFLVDS 

       370        380        390        400        410        420 
PLIDVQPSGD EKVPPCGFGS FHQQYRIDGR LIAVGVVDIL PKCLSSVYLF WDPDYAFLSL 

       430        440        450        460        470        480 
GKYSAIQEIN WVIENQARCP SLQYYYLGYY IHSCSKMRYK AAYRPSELLC PLRFQWVPFE 

       490        500        510        520        530        540 
VARPMLDKKP YVILSDIAIS HNQCSLLAGA SETLVEPAAS EHEDMEQGET NDNFMGCSDE 

       550        560        570        580        590        600 
DEDEDEDDDD DDDDDEEMYE TESEDSHIES DPGSKDNDIN NILIGLYGSQ YRYKEMRQII 

       610        620        630 
TPVGRKQLEP MLQSYRKVVG AELSERMVYE IN 

References and documentation are available.

Number of amino acids: 632

Molecular weight: 71541.11

Theoretical pI: 5.58

Amino acid composition: 
Ala (A)  36	  5.7%
Arg (R)  41	  6.5%
Asn (N)  23	  3.6%
Asp (D)  51	  8.1%
Cys (C)  14	  2.2%
Gln (Q)  26	  4.1%
Glu (E)  50	  7.9%
Gly (G)  36	  5.7%
His (H)  17	  2.7%
Ile (I)  36	  5.7%
Leu (L)  47	  7.4%
Lys (K)  43	  6.8%
Met (M)  14	  2.2%
Phe (F)  16	  2.5%
Pro (P)  29	  4.6%
Ser (S)  67	 10.6%
Thr (T)  19	  3.0%
Trp (W)   4	  0.6%
Tyr (Y)  28	  4.4%
Val (V)  35	  5.5%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 101
Total number of positively charged residues (Arg + Lys): 84

Atomic composition:

Carbon      C	      3109
Hydrogen    H	      4899
Nitrogen    N	       885
Oxygen      O	       998
Sulfur      S	        28

Formula: C3109H4899N885O998S28
Total number of atoms: 9919

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    64595
Abs 0.1% (=1 g/l)   0.903, assuming all pairs of Cys residues form cystines


Ext. coefficient    63720
Abs 0.1% (=1 g/l)   0.891, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 49.91
This classifies the protein as unstable.



Aliphatic index: 72.97

Grand average of hydropathicity (GRAVY): -0.697