ProtParam
User-provided sequence:
10 20 30 40 50 60
MSLLLPTNLQ QYPSSSSFPS STPILSPPPS TAFSVIVPRR RCLRLVTSCV STVQSSVATN
70 80 90 100 110 120
GSSPAPAPAA VVVERDQIRL GLPSKGRMAA DAIDLLKDCQ LFVKQVNPRQ YVAQIPQLPN
130 140 150 160 170 180
TEVWFQRPKD IVRKLLSGDL DLGIVGLDTL SEYGQENEDL IIVHEALNFG DCHLSIAIPN
190 200 210 220 230 240
YGIFENINSL KELAQMPQWS EERPLRLATG FTYLGPKFMK ENGIKHVVFS TADGALEAAP
250 260 270 280 290 300
AMGIADAILD LVSSGITLKE NNLKEIEGGV VLESQAALVA SRRALNERKG ALNTVHEILE
310 320 330 340 350 360
RLEAHLKADG QFTVVANMRG NSAQEVAERV LSQPSLSGLQ GPTISPVYCT QNGKVSVDYY
370 380 390 400 410
AIVICVPKKA LYDSVKQLRA AGGSGVLVSP LTYIFDEDTP RWGQLLRNLG I
References and
documentation are available.
Number of amino acids: 411
Molecular weight: 44556.16
Theoretical pI: 6.04
Total number of negatively charged residues (Asp + Glu): 41
Total number of positively charged residues (Arg + Lys): 38
Atomic composition:
Carbon C 1981
Hydrogen H 3195
Nitrogen N 543
Oxygen O 597
Sulfur S 12
Formula: C
1981H
3195N
543O
597S
12
Total number of atoms: 6328
Extinction coefficients:
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 31775
Abs 0.1% (=1 g/l) 0.713, assuming all pairs of Cys residues form cystines
Ext. coefficient 31400
Abs 0.1% (=1 g/l) 0.705, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 46.92
This classifies the protein as unstable.
Aliphatic index: 102.48
Grand average of hydropathicity (GRAVY): -0.017