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ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MSLLRIRKPK TRKGKKVLLA REPQLIESAR TMLFLDGRKC GGNVKLCMKD LQALKKPLVK

70 80 90 100 110 120
VLNRKNDITP FDDPSSLEFL TMKNDAALFT FGSTSKKRPD NIILGRIFEN EVLDMFELGI

130 140 150 160 170 180
KRYQAISEFK NEKIGACVKP CLVFNGPKWA QTEELRRLRN LFIDTFQREK VDSIRLQGIE

190 200 210 220 230 240
HVLSFTVIDD MNILMRSYRI LLKKSGQRTP RIELEEIGPS ADFSIRRTKI ASEDLYKQAR

250 260 270 280 290 300
KQPKQLKVGK KKNISTDALG NTKGRVHLGK QQTGSIQTRR VKALRKTPEE KKENRQRKKV

310 320
ALKAAAAEAL ASQGNNPFSS


References and documentation are available.

Number of amino acids: 320 Molecular weight: 36509.76 Theoretical pI: 10.26
Amino acid composition: 
Ala (A) 20 6.2% Arg (R) 28 8.8% Asn (N) 15 4.7% Asp (D) 15 4.7% Cys (C) 4 1.2% Gln (Q) 15 4.7% Glu (E) 20 6.2% Gly (G) 18 5.6% His (H) 2 0.6% Ile (I) 21 6.6% Leu (L) 35 10.9% Lys (K) 40 12.5% Met (M) 7 2.2% Phe (F) 14 4.4% Pro (P) 13 4.1% Ser (S) 19 5.9% Thr (T) 16 5.0% Trp (W) 1 0.3% Tyr (Y) 3 0.9% Val (V) 14 4.4% Pyl (O) 0 0.0% Sec (U) 0 0.0% (B) 0 0.0% (Z) 0 0.0% (X) 0 0.0%
Total number of negatively charged residues (Asp + Glu): 35 Total number of positively charged residues (Arg + Lys): 68 Atomic composition: Carbon C 1614 Hydrogen H 2697 Nitrogen N 479 Oxygen O 459 Sulfur S 11 Formula: C1614H2697N479O459S11 Total number of atoms: 5260 Extinction coefficients: Extinction coefficients are in units of M-1 cm-1, at 280 nm measured in water. Ext. coefficient 10220 Abs 0.1% (=1 g/l) 0.280, assuming all pairs of Cys residues form cystines Ext. coefficient 9970 Abs 0.1% (=1 g/l) 0.273, assuming all Cys residues are reduced Estimated half-life: The N-terminal of the sequence considered is M (Met). The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro). >20 hours (yeast, in vivo). >10 hours (Escherichia coli, in vivo). Instability index: The instability index (II) is computed to be 44.78 This classifies the protein as unstable. Aliphatic index: 87.19 Grand average of hydropathicity (GRAVY): -0.595