ProtParam
User-provided sequence:
10 20 30 40 50 60
MSLLRIRKPK TRKGKKVLLA REPQLIESAR TMLFLDGRKC GGNVKLCMKD LQALKKPLVK
70 80 90 100 110 120
VLNRKNDITP FDDPSSLEFL TMKNDAALFT FGSTSKKRPD NIILGRIFEN EVLDMFELGI
130 140 150 160 170 180
KRYQAISEFK NEKIGACVKP CLVFNGPKWA QTEELRRLRN LFIDTFQREK VDSIRLQGIE
190 200 210 220 230 240
HVLSFTVIDD MNILMRSYRI LLKKSGQRTP RIELEEIGPS ADFSIRRTKI ASEDLYKQAR
250 260 270 280 290 300
KQPKQLKVGK KKNISTDALG NTKGRVHLGK QQTGSIQTRR VKALRKTPEE KKENRQRKKV
310 320
ALKAAAAEAL ASQGNNPFSS
References and
documentation are available.
Number of amino acids: 320
Molecular weight: 36509.76
Theoretical pI: 10.26
Total number of negatively charged residues (Asp + Glu): 35
Total number of positively charged residues (Arg + Lys): 68
Atomic composition:
Carbon C 1614
Hydrogen H 2697
Nitrogen N 479
Oxygen O 459
Sulfur S 11
Formula: C
1614H
2697N
479O
459S
11
Total number of atoms: 5260
Extinction coefficients:
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 10220
Abs 0.1% (=1 g/l) 0.280, assuming all pairs of Cys residues form cystines
Ext. coefficient 9970
Abs 0.1% (=1 g/l) 0.273, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 44.78
This classifies the protein as unstable.
Aliphatic index: 87.19
Grand average of hydropathicity (GRAVY): -0.595