ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MSLNYSLLDK FHSYYERRAH NNNELTTKLV KRVVIISGIF LFCGIVSYAL TTFVFHFYKE 

        70         80         90        100        110        120 
DKFSKNEVGL DPICKRRIVG YYSEYDSTDI SKNQLAKLTH AVFAFVDIKY DGTLQFKNLI 

       130        140        150        160        170        180 
TEQKFFSLKS KARSLHSNLK LMFSIGGDEN SFDFSSALAN TQMKSTLITS IIAFIHSHMI 

       190        200        210        220        230        240 
DGVDLHWKWP TSRDKSNYAT LIREIREKVD ELDAKIIISI TIPPVGVSDW ESGFDLDAIQ 

       250        260        270        280        290        300 
KHVDFINVHS MDYAKPLPNQ WGTPTGPSAS MNFNIGLRQH YNVDWTMKHY TCELKKPSMI 

       310        320        330        340        350        360 
NLVIPFYVRM WKNVQKAIDN RTEVFRNVEL KDNEVEGRSQ LSRYTVEHED MELSPESWDN 

       370        380        390        400        410        420 
ATQTPYVLDL KTRTFFTYEN EKSIKVKLDY VNKMDLGGVW IWSVDMDDRL NTLLSFVFPN 

       430 
ELCSSYSENG IKYSC 

References and documentation are available.

Number of amino acids: 435

Molecular weight: 50487.56

Theoretical pI: 6.51

Amino acid composition: 
Ala (A)  16	  3.7%
Arg (R)  17	  3.9%
Asn (N)  27	  6.2%
Asp (D)  30	  6.9%
Cys (C)   5	  1.1%
Gln (Q)  10	  2.3%
Glu (E)  24	  5.5%
Gly (G)  17	  3.9%
His (H)  13	  3.0%
Ile (I)  31	  7.1%
Leu (L)  37	  8.5%
Lys (K)  34	  7.8%
Met (M)  12	  2.8%
Phe (F)  25	  5.7%
Pro (P)  13	  3.0%
Ser (S)  40	  9.2%
Thr (T)  26	  6.0%
Trp (W)   9	  2.1%
Tyr (Y)  20	  4.6%
Val (V)  29	  6.7%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 54
Total number of positively charged residues (Arg + Lys): 51

Atomic composition:

Carbon      C	      2285
Hydrogen    H	      3506
Nitrogen    N	       592
Oxygen      O	       667
Sulfur      S	        17

Formula: C2285H3506N592O667S17
Total number of atoms: 7067

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    79550
Abs 0.1% (=1 g/l)   1.576, assuming all pairs of Cys residues form cystines


Ext. coefficient    79300
Abs 0.1% (=1 g/l)   1.571, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 29.52
This classifies the protein as stable.



Aliphatic index: 83.98

Grand average of hydropathicity (GRAVY): -0.334