ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MSLPATFDLT PEDAQLLLAA NTHLGARNVQ VHQEPYVFNA RPDGVHVINV GKTWEKLVLA 

        70         80         90        100        110        120 
ARIIAAIPNP EDVVAISSRT YGQRAVLKFA AHTGATPIAG RFTPGSFTNY ITRSFKEPRL 

       130        140        150        160        170        180 
VIVTDPRLDA QAIKEASYVN IPVIALTDLD SPSEFVDVAI PCNNRGKHSI GLIWYLLARE 

       190        200        210        220        230        240 
VLRLRGALVD RTQPWSIMPD LYFYRNPEEV EQVAEEAAAA EEGEEEEVKE EVTEGQAEAT 

       250 
EWAEENADNV EW 

References and documentation are available.

Number of amino acids: 252

Molecular weight: 27962.46

Theoretical pI: 4.69

Amino acid composition: 
Ala (A)  32	 12.7%
Arg (R)  15	  6.0%
Asn (N)  12	  4.8%
Asp (D)  12	  4.8%
Cys (C)   1	  0.4%
Gln (Q)   8	  3.2%
Glu (E)  27	 10.7%
Gly (G)  12	  4.8%
His (H)   5	  2.0%
Ile (I)  15	  6.0%
Leu (L)  20	  7.9%
Lys (K)   7	  2.8%
Met (M)   2	  0.8%
Phe (F)   8	  3.2%
Pro (P)  16	  6.3%
Ser (S)  10	  4.0%
Thr (T)  15	  6.0%
Trp (W)   5	  2.0%
Tyr (Y)   7	  2.8%
Val (V)  23	  9.1%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 39
Total number of positively charged residues (Arg + Lys): 22

Atomic composition:

Carbon      C	      1251
Hydrogen    H	      1949
Nitrogen    N	       339
Oxygen      O	       383
Sulfur      S	         3

Formula: C1251H1949N339O383S3
Total number of atoms: 3925

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    37930
Abs 0.1% (=1 g/l)   1.356, assuming all pairs of Cys residues form cystines


Ext. coefficient    37930
Abs 0.1% (=1 g/l)   1.356, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 33.70
This classifies the protein as stable.



Aliphatic index: 93.33

Grand average of hydropathicity (GRAVY): -0.212