ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MSLPIIKVHW LDHSRAFRLL WLLDHLNLEY EIVPYKRDAN FRAPPELKKI HPLGRSPLLE 

        70         80         90        100        110        120 
VQDRETGKKK ILAESGFIFQ YVLQHFDHSH VLMSEDADIA DQINYYLFYV EGSLQPPLMI 

       130        140        150        160        170        180 
EFILSKVKDS GMPFPISYLA RKVADKISQA YSSGEVKNQF DFVEGEISKN NGYLVDGKLS 

       190        200        210        220        230 
GADILMSFPL QMAFERKFAA PEDYPAISKW LKTITSEESY AASKEKARAL GSNF 

References and documentation are available.

Number of amino acids: 234

Molecular weight: 26794.80

Theoretical pI: 6.18

Amino acid composition: 
Ala (A)  18	  7.7%
Arg (R)   9	  3.8%
Asn (N)   7	  3.0%
Asp (D)  14	  6.0%
Cys (C)   0	  0.0%
Gln (Q)   8	  3.4%
Glu (E)  17	  7.3%
Gly (G)  11	  4.7%
His (H)   7	  3.0%
Ile (I)  16	  6.8%
Leu (L)  26	 11.1%
Lys (K)  19	  8.1%
Met (M)   6	  2.6%
Phe (F)  14	  6.0%
Pro (P)  13	  5.6%
Ser (S)  21	  9.0%
Thr (T)   3	  1.3%
Trp (W)   3	  1.3%
Tyr (Y)  11	  4.7%
Val (V)  11	  4.7%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 31
Total number of positively charged residues (Arg + Lys): 28

Atomic composition:

Carbon      C	      1230
Hydrogen    H	      1892
Nitrogen    N	       312
Oxygen      O	       347
Sulfur      S	         6

Formula: C1230H1892N312O347S6
Total number of atoms: 3787

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    32890
Abs 0.1% (=1 g/l)   1.227

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 48.24
This classifies the protein as unstable.



Aliphatic index: 91.32

Grand average of hydropathicity (GRAVY): -0.253