ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MSLQFSYEKY MNLIQYPEHW SNPIPHEISK KVIYNTLSPC ICVQSNLELD NHLKDMYNID 

        70         80         90        100        110        120 
SISMLFRYFG DYIQDRDQIP STTDLKDPGS VSSGRHRSDS LFQRQASECV RFTRSLEDLI 

       130        140        150        160        170        180 
SIDSEDHILQ STDIDSFLSQ YLQNLEPIMN ENGTPSKLLK HSIYHRFFIT LSSINLSRYH 

       190        200        210        220        230        240 
SFHHPVVALL PLDITKGQGY ENAREILIHF KNLNNTLENF PEFININDIL PVFVLCYDEN 

       250        260        270        280        290        300 
SREQWESVQA LTKSLKKQLF VESVSISLFT PHDNQKSTTL HSPITVSLHE QVFNMSHPTS 

       310        320        330        340        350        360 
INIPSRLLTH IYDTISFIVE EMMIPFMHRK LVFWDETVLQ PRKSIFQSNK FFRRFISPKS 

       370        380        390        400        410        420 
NHPIAHKVND PQDPAEGDIM FPASSPEFLL RKLADWSFML SDFKKAYSIY DLLTKDFESY 

       430        440        450        460        470        480 
PQYLAPCLEY KTISLLMGAQ NIITAKTIKT EVDPLMTRAI EQYKKQDEKH SLRVIHCILT 

       490        500        510        520        530        540 
FSDLLLSLSD TWVSSPLAVN YLTYIQKSHL LGPYATVIIL ERIAYAYEIC IDLRVNDHFL 

       550        560        570        580        590        600 
PKPDEENKAD ESEAAENSKK GENGLEVDDM HEWRNENKLI QNNLRSEGLT RFRKYSLYQL 

       610        620        630        640 
VAAKKWVELH KRNQAKWCIY HAAPAYEKLS LSKRPDGLFH RLSMELS 

References and documentation are available.

Number of amino acids: 647

Molecular weight: 75371.99

Theoretical pI: 6.14

Amino acid composition: 
Ala (A)  26	  4.0%
Arg (R)  28	  4.3%
Asn (N)  35	  5.4%
Asp (D)  38	  5.9%
Cys (C)   8	  1.2%
Gln (Q)  27	  4.2%
Glu (E)  43	  6.6%
Gly (G)  13	  2.0%
His (H)  27	  4.2%
Ile (I)  51	  7.9%
Leu (L)  73	 11.3%
Lys (K)  41	  6.3%
Met (M)  15	  2.3%
Phe (F)  32	  4.9%
Pro (P)  33	  5.1%
Ser (S)  66	 10.2%
Thr (T)  29	  4.5%
Trp (W)   8	  1.2%
Tyr (Y)  28	  4.3%
Val (V)  26	  4.0%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 81
Total number of positively charged residues (Arg + Lys): 69

Atomic composition:

Carbon      C	      3402
Hydrogen    H	      5262
Nitrogen    N	       896
Oxygen      O	       995
Sulfur      S	        23

Formula: C3402H5262N896O995S23
Total number of atoms: 10578

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    86220
Abs 0.1% (=1 g/l)   1.144, assuming all pairs of Cys residues form cystines


Ext. coefficient    85720
Abs 0.1% (=1 g/l)   1.137, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 48.64
This classifies the protein as unstable.



Aliphatic index: 90.42

Grand average of hydropathicity (GRAVY): -0.381