ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MSLQLLNPKA ESLRRDAALK VNVTSAEGLQ SVLETNLGPK GTLKMLVDGA GNIKLTKDGK 

        70         80         90        100        110        120 
VLLTEMQIQS PTAVLIARAA AAQDEITGDG TTTVVCLVGE LLRQAHRFIQ EGVHPRIITD 

       130        140        150        160        170        180 
GFEIARKESM KFLDEFKISK TNLSNDREFL LQVARSSLLT KVDADLTEVL TPIVTDAVLS 

       190        200        210        220        230        240 
VYDAQADNLD LHMVEIMQMQ HLSPKDTTFI KGLVLDHGGR HPDMPTRVKN AYVLILNVSL 

       250        260        270        280        290        300 
EYEKTEVNSG FFYSSADQRD KLAASERKFV DAKLKKIIDL KNEVCGMDPD KGFVIINQKG 

       310        320        330        340        350        360 
IDPMSLDVFA KHNILALRRA KRRNMERLQL VTGGEAQNSV EDLSPQILGF SGLVYQETIG 

       370        380        390        400        410        420 
EEKFTYVTEN TDPKSCTILI KGSTHYALAQ TKDAVRDGLR AVANVLKDKN IIPGAGAFYI 

       430        440        450        460        470        480 
ALSRYLRSAN MNKLGAKGKT KTGIEAFAEA LLVIPKTLVK NSGFDPLDVL AMVEDELDDA 

       490        500        510        520        530        540 
QDSDETRYVG VDLNIGDSCD PTIEGIWDSY RVLRNAITGA TGIASNLLLC DELLRAGRST 


LKETPQ 

References and documentation are available.

Number of amino acids: 546

Molecular weight: 59923.73

Theoretical pI: 5.60

Amino acid composition: 
Ala (A)  46	  8.4%
Arg (R)  27	  4.9%
Asn (N)  24	  4.4%
Asp (D)  42	  7.7%
Cys (C)   5	  0.9%
Gln (Q)  20	  3.7%
Glu (E)  33	  6.0%
Gly (G)  37	  6.8%
His (H)   8	  1.5%
Ile (I)  33	  6.0%
Leu (L)  66	 12.1%
Lys (K)  39	  7.1%
Met (M)  13	  2.4%
Phe (F)  16	  2.9%
Pro (P)  17	  3.1%
Ser (S)  31	  5.7%
Thr (T)  37	  6.8%
Trp (W)   1	  0.2%
Tyr (Y)  11	  2.0%
Val (V)  40	  7.3%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 75
Total number of positively charged residues (Arg + Lys): 66

Atomic composition:

Carbon      C	      2639
Hydrogen    H	      4313
Nitrogen    N	       727
Oxygen      O	       820
Sulfur      S	        18

Formula: C2639H4313N727O820S18
Total number of atoms: 8517

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    22140
Abs 0.1% (=1 g/l)   0.369, assuming all pairs of Cys residues form cystines


Ext. coefficient    21890
Abs 0.1% (=1 g/l)   0.365, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 37.40
This classifies the protein as stable.



Aliphatic index: 100.38

Grand average of hydropathicity (GRAVY): -0.168