ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MSLREVTNYE VSFYIPLSYS NRTHKVCKLP NGILALIISD PTDTSSSCSL TVCTGSHNDP 

        70         80         90        100        110        120 
KDIAGLAHLC EHMILSAGSK KYPDPGLFHT LIAKNNGSQN AFTTGEQTTF YFELPNTQNN 

       130        140        150        160        170        180 
GEFTFESILD VFASFFKEPL FNPLLISKEI YAIQSEHEGN ISSTTKIFYH AARILANPDH 

       190        200        210        220        230        240 
PFSRFSTGNI HSLSSIPQLK KIKLKSSLNT YFENNFFGEN ITLCIRGPQS VNILTKLALS 

       250        260        270        280        290        300 
KFGDIKPKSA VKERSISIRT RSFRRSKSLK KRQDSSKNDY SDLKTFKILN TTWEKKYKNT 

       310        320        330        340        350        360 
MCFQQFPECN SIFINSNKVP IMRLLFPVSD KNTRFTKDDI KIYSHLWCEL FGDESPGSLS 

       370        380        390        400        410        420 
YYLASKGWLT GCFAFTSEFA IGDIGLILEL ELTNSGWENI KRITTIVLNR LLPSFYVMNI 

       430        440        450        460        470        480 
DYLITFLKEQ NLIDLVSFLY QSSEDLPMEE CSKLSGILQD DLECLTPPNI FKGFKSLIEI 

       490        500        510        520        530        540 
DDPNIEKYEN TKANIQWWTG QAIKFQNFLK SFMNHDNMRL LLLGNIKSGN IFDKMKNKSD 

       550        560        570        580        590        600 
ICTDFFYEFE YYTANVHLAS DNKFHSNSSY EFNFPTGNLF LPDCVSDPLK LQQLFLECSL 

       610        620        630        640        650        660 
KSKFATLRPQ IYSEPTRTKP QLVSENQNYE MWILKEDPNF ASDNKSVVSF EVLGLGIKPS 

       670        680        690        700        710        720 
PEATIHLEVL AQALFIITSS FLYPALRIGY TYEIASSSKG NVTLRFTISG FPEGVFTIVK 

       730        740        750        760        770        780 
TFVDTLKLIA TDPTFLSKDT LRKARILVRN KYKNASSDNC VKLASVGLLI VLEKYIWTLE 

       790        800        810        820        830        840 
DRINALELTE LESFEKFCFL FWRNPKHLVL FMQGSLEYAD AINRYLNNNF TQHLKISNEG 

       850        860        870        880        890        900 
SKPTIRLYPP PSTKDLDQGT NAFISYNGHQ DDPNNSIVYF IQTAQRDDIK NLTLTFLTEY 

       910        920        930        940        950        960 
LFSLTLVPDL RNKKQIGYIV LGGLRVLTDT VGIHITVMSG SSGHNLETRI NEYLSYLQLQ 

       970        980        990       1000       1010       1020 
VLNRFTEFDF RRILLEPFLN LLKQNSTKQF EGSAGPVDLL NEIVANVQNG DNYTLNNKQM 

      1030       1040       1050       1060       1070       1080 
RQHRKVRNKI AEGRLNFQED HEMIDISFLQ KLTLKKYLAF FESKISIYSA QRSKLSIMIT 

      1090       1100       1110       1120       1130       1140 
SPMAEKEIAS RKMFLQLEAF LKINGFAIKN EDLKKIVEHS KGNPILLVKN LFTYFRRRNE 

      1150       1160       1170       1180       1190       1200 
VFKLGTVVLQ EILKIIGMNL KQRYGSILGF SSQDGEGQEI EKFWNNDTSP IVPLQELPEP 


NFFRKAAF 

References and documentation are available.

Number of amino acids: 1208

Molecular weight: 138331.70

Theoretical pI: 8.56

Amino acid composition: 
Ala (A)  47	  3.9%
Arg (R)  45	  3.7%
Asn (N)  89	  7.4%
Asp (D)  54	  4.5%
Cys (C)  16	  1.3%
Gln (Q)  44	  3.6%
Glu (E)  74	  6.1%
Gly (G)  55	  4.6%
His (H)  22	  1.8%
Ile (I)  95	  7.9%
Leu (L) 141	 11.7%
Lys (K)  93	  7.7%
Met (M)  18	  1.5%
Phe (F)  86	  7.1%
Pro (P)  50	  4.1%
Ser (S) 107	  8.9%
Thr (T)  75	  6.2%
Trp (W)  10	  0.8%
Tyr (Y)  42	  3.5%
Val (V)  45	  3.7%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 128
Total number of positively charged residues (Arg + Lys): 138

Atomic composition:

Carbon      C	      6285
Hydrogen    H	      9791
Nitrogen    N	      1623
Oxygen      O	      1822
Sulfur      S	        34

Formula: C6285H9791N1623O1822S34
Total number of atoms: 19555

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient   118580
Abs 0.1% (=1 g/l)   0.857, assuming all pairs of Cys residues form cystines


Ext. coefficient   117580
Abs 0.1% (=1 g/l)   0.850, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 40.45
This classifies the protein as unstable.



Aliphatic index: 90.89

Grand average of hydropathicity (GRAVY): -0.249