ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MSLRIKALDA SVVNKIAAGE IIISPVNALK EMMENSIDAN ATMIDILVKE GGIKVLQITD 

        70         80         90        100        110        120 
NGSGINKADL PILCERFTTS KLQKFEDLSQ IQTYGFRGEA LASISHVARV TVTTKVKEDR 

       130        140        150        160        170        180 
CAWRVSYAEG KMLESPKPVA GKDGTTILVE DLFFNIPSRL RALRSHNDEY SKILDVVGRY 

       190        200        210        220        230        240 
AIHSKDIGFS CKKFGDSNYS LSVKPSYTVQ DRIRTVFNKS VASNLITFHI SKVEDLNLES 

       250        260        270        280        290        300 
VDGKVCNLNF ISKKSISPIF FINNRLVTCD LLRRALNSVY SNYLPKGNRP FIYLGIVIDP 

       310        320        330        340        350        360 
AAVDVNVHPT KREVRFLSQD EIIEKIANQL HAELSAIDTS RTFKASSIST NKPESLIPFN 

       370        380        390        400        410        420 
DTIESDRNRK SLRQAQVVEN SYTTANSQLR KAKRQENKLV RIDASQAKIT SFLSSSQQFN 

       430        440        450        460        470        480 
FEGSSTKRQL SEPKVTNVSH SQEAEKLTLN ESEQPRDANT INDNDLKDQP KKKQKLGDYK 

       490        500        510        520        530        540 
VPSIADDEKN ALPISKDGYI RVPKERVNVN LTSIKKLREK VDDSIHRELT DIFANLNYVG 

       550        560        570        580        590        600 
VVDEERRLAA IQHDLKLFLI DYGSVCYELF YQIGLTDFAN FGKINLQSTN VSDDIVLYNL 

       610        620        630        640        650        660 
LSEFDELNDD ASKEKIISKI WDMSSMLNEY YSIELVNDGL DNDLKSVKLK SLPLLLKGYI 

       670        680        690        700        710        720 
PSLVKLPFFI YRLGKEVDWE DEQECLDGIL REIALLYIPD MVPKVDTSDA SLSEDEKAQF 

       730        740        750        760 
INRKEHISSL LEHVLFPCIK RRFLAPRHIL KDVVEIANLP DLYKVFERC 

References and documentation are available.

Number of amino acids: 769

Molecular weight: 87062.48

Theoretical pI: 6.52

Amino acid composition: 
Ala (A)  43	  5.6%
Arg (R)  40	  5.2%
Asn (N)  48	  6.2%
Asp (D)  57	  7.4%
Cys (C)   9	  1.2%
Gln (Q)  24	  3.1%
Glu (E)  51	  6.6%
Gly (G)  27	  3.5%
His (H)  12	  1.6%
Ile (I)  64	  8.3%
Leu (L)  80	 10.4%
Lys (K)  65	  8.5%
Met (M)   8	  1.0%
Phe (F)  31	  4.0%
Pro (P)  27	  3.5%
Ser (S)  72	  9.4%
Thr (T)  31	  4.0%
Trp (W)   3	  0.4%
Tyr (Y)  23	  3.0%
Val (V)  54	  7.0%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 108
Total number of positively charged residues (Arg + Lys): 105

Atomic composition:

Carbon      C	      3875
Hydrogen    H	      6233
Nitrogen    N	      1053
Oxygen      O	      1184
Sulfur      S	        17

Formula: C3875H6233N1053O1184S17
Total number of atoms: 12362

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    51270
Abs 0.1% (=1 g/l)   0.589, assuming all pairs of Cys residues form cystines


Ext. coefficient    50770
Abs 0.1% (=1 g/l)   0.583, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 38.28
This classifies the protein as stable.



Aliphatic index: 98.99

Grand average of hydropathicity (GRAVY): -0.321