ProtParam
User-provided sequence:
10 20 30 40 50 60
MSLSLGAAIY IALKPIFKIY TIMLVGYLVA KFDIVSMENA KGISNMVVNA ILPCLTFNKI
70 80 90 100 110 120
VSNISWRDIK EIGVIILSAF ILFVLGATGA LFTTFATTVP KKFFWGLIFA GFFPNISDLP
130 140 150 160 170 180
IAYIQSMGNG SIFTAEEADK GVAYSCIFLF IQSFLMMNFG MWRVVGLDFR DTKEPDSENI
190 200 210 220 230 240
TPSVSPAIDD RKLTEITKLP NITRPTNAYQ SEDARSNSDL SCNSITTNEM TPQAFYEGFT
250 260 270 280 290 300
GYIKPYKESN GASHKFESDL PHAEIYRVSS TYSSPGALEF SRIDGSSLSY SRISKNSDGR
310 320 330 340 350 360
SYRRKRKADM NELISKYSAA EKIRQGELDL SRPLSLTEEV GSRNASIGNV HTGYTDESSI
370 380 390 400 410 420
EEENCTNMAT DGRGSLSFFI ERHNLKWLQY FIINCLRPAS LGAILGIICA LIPWVKACFV
430 440 450 460 470 480
TTYVHVHKAP DGEPVLNFLM DFTEYIGNAC VPLGLLLLGG TLARLEIKSL PPGFIKSALL
490 500 510 520 530 540
MTCFRLIVIP IIGVLWVNKL YSIDWLDTGI GKFDMILTWS MPSATAQVYF TAFYTPACGD
550 560 570
HIQMNCLSVL FVMQYAILFI TVAFVVTYTL KVDLKV
References and
documentation are available.
Number of amino acids: 576
Molecular weight: 64032.31
Theoretical pI: 6.42
Total number of negatively charged residues (Asp + Glu): 52
Total number of positively charged residues (Arg + Lys): 50
Atomic composition:
Carbon C 2924
Hydrogen H 4546
Nitrogen N 726
Oxygen O 829
Sulfur S 28
Formula: C
2924H
4546N
726O
829S
28
Total number of atoms: 9053
Extinction coefficients:
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 78895
Abs 0.1% (=1 g/l) 1.232, assuming all pairs of Cys residues form cystines
Ext. coefficient 78270
Abs 0.1% (=1 g/l) 1.222, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 40.07
This classifies the protein as unstable.
Aliphatic index: 98.89
Grand average of hydropathicity (GRAVY): 0.236