ProtParam
User-provided sequence:
10 20 30 40 50 60
MSLSLVSYRL RKNPWVNIFL PVLAIFLIYI IFFQRDQSLL GLNGQSISQH KWAHEKENTF
70 80 90 100 110 120
YFPFTKKYKM PKYSYKKKSG WLFNDHVEDI IPEGHIAHYD LNKLHSTSEA AVNKEHILIL
130 140 150 160 170 180
TPMQTFHQQY WDNLLQLNYP RELIELGFIT PRTATGDLAL KKLENAIKKV QTDKKTQRFS
190 200 210 220 230 240
KITILRQNSQ SFDKLMEKER HALDVQKERR AAMALARNEL LFSTIGPHTS WVLWLDADII
250 260 270 280 290 300
ETPPSLIQDM TKHNKAILAA NIYQRFYDEE KKQPSIRPYD FNNWQESDTG LEIASQMGDD
310 320 330 340 350 360
EIIVEGYAEI ATYRPLMAHF YDANGVPGEE MALDGVGGGC TLVKAEVHRD GAMFPNFPFY
370 380 390
HLIETEGFAK MAKRLNYDVF GLPNYLVYHI EEENH
References and
documentation are available.
Number of amino acids: 395
Molecular weight: 45954.55
Theoretical pI: 6.36
Total number of negatively charged residues (Asp + Glu): 50
Total number of positively charged residues (Arg + Lys): 45
Atomic composition:
Carbon C 2095
Hydrogen H 3206
Nitrogen N 550
Oxygen O 592
Sulfur S 12
Formula: C
2095H
3206N
550O
592S
12
Total number of atoms: 6455
Extinction coefficients:
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 66810
Abs 0.1% (=1 g/l) 1.454, assuming all pairs of Cys residues form cystines
Ext. coefficient 66810
Abs 0.1% (=1 g/l) 1.454, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 58.89
This classifies the protein as unstable.
Aliphatic index: 85.97
Grand average of hydropathicity (GRAVY): -0.424