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ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MSLSLVSYRL RKNPWVNIFL PVLAIFLIYI IFFQRDQSLL GLNGQSISQH KWAHEKENTF

70 80 90 100 110 120
YFPFTKKYKM PKYSYKKKSG WLFNDHVEDI IPEGHIAHYD LNKLHSTSEA AVNKEHILIL

130 140 150 160 170 180
TPMQTFHQQY WDNLLQLNYP RELIELGFIT PRTATGDLAL KKLENAIKKV QTDKKTQRFS

190 200 210 220 230 240
KITILRQNSQ SFDKLMEKER HALDVQKERR AAMALARNEL LFSTIGPHTS WVLWLDADII

250 260 270 280 290 300
ETPPSLIQDM TKHNKAILAA NIYQRFYDEE KKQPSIRPYD FNNWQESDTG LEIASQMGDD

310 320 330 340 350 360
EIIVEGYAEI ATYRPLMAHF YDANGVPGEE MALDGVGGGC TLVKAEVHRD GAMFPNFPFY

370 380 390
HLIETEGFAK MAKRLNYDVF GLPNYLVYHI EEENH


References and documentation are available.

Number of amino acids: 395 Molecular weight: 45954.55 Theoretical pI: 6.36
Amino acid composition: 
Ala (A) 27 6.8% Arg (R) 16 4.1% Asn (N) 21 5.3% Asp (D) 21 5.3% Cys (C) 1 0.3% Gln (Q) 18 4.6% Glu (E) 29 7.3% Gly (G) 19 4.8% His (H) 16 4.1% Ile (I) 29 7.3% Leu (L) 40 10.1% Lys (K) 29 7.3% Met (M) 11 2.8% Phe (F) 21 5.3% Pro (P) 18 4.6% Ser (S) 19 4.8% Thr (T) 19 4.8% Trp (W) 7 1.8% Tyr (Y) 19 4.8% Val (V) 15 3.8% Pyl (O) 0 0.0% Sec (U) 0 0.0% (B) 0 0.0% (Z) 0 0.0% (X) 0 0.0%
Total number of negatively charged residues (Asp + Glu): 50 Total number of positively charged residues (Arg + Lys): 45 Atomic composition: Carbon C 2095 Hydrogen H 3206 Nitrogen N 550 Oxygen O 592 Sulfur S 12 Formula: C2095H3206N550O592S12 Total number of atoms: 6455 Extinction coefficients: Extinction coefficients are in units of M-1 cm-1, at 280 nm measured in water. Ext. coefficient 66810 Abs 0.1% (=1 g/l) 1.454, assuming all pairs of Cys residues form cystines Ext. coefficient 66810 Abs 0.1% (=1 g/l) 1.454, assuming all Cys residues are reduced Estimated half-life: The N-terminal of the sequence considered is M (Met). The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro). >20 hours (yeast, in vivo). >10 hours (Escherichia coli, in vivo). Instability index: The instability index (II) is computed to be 58.89 This classifies the protein as unstable. Aliphatic index: 85.97 Grand average of hydropathicity (GRAVY): -0.424