ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MSLSNKTVKE LRQLLKERYT VEDELTESIA LSSMRFKPSQ EPEFHALSQS SLLKTKLKQQ 

        70         80         90        100        110        120 
SSTDIPSYTH VLISKLSCEK ITHYAVRGGN IEIMGILMGF TLKDNIVVMD CFNLPVVGTE 

       130        140        150        160        170        180 
TRVNAQLESY EYMVQYIDEM YNHNDGGDGR DYKGAKLNVV GWFHSHPGYD CWLSNIDIQT 

       190        200        210        220        230        240 
QDLNQRFQDP YVAIVVDPLK SLEDKILRMG AFRTIESKSD DNSATSYYEL ETIIFDSELN 

       250        260        270        280        290        300 
RALFETKLNL HCVIEDDESE QISLNRLIDS MKQYSYLMDS KNVRTRIKLA TTSERVSNEN 

       310        320        330        340        350        360 
KKNIDYQNRS TRSQFCLNTQ RGDSTETSSF GSMFSGDNTS DVDMEDRNLT EFDSTDTSLC 

       370        380        390        400        410        420 
INGEPSIHVN RVERSSRSTD NFHNSKKRMN SNQERCHDEG NDMLQRNVLE TDYARAKNRI 

       430        440 
LASKIKQYER LRFYKDTFTL 

References and documentation are available.

Number of amino acids: 440

Molecular weight: 50811.78

Theoretical pI: 5.45

Amino acid composition: 
Ala (A)  13	  3.0%
Arg (R)  28	  6.4%
Asn (N)  31	  7.0%
Asp (D)  34	  7.7%
Cys (C)   7	  1.6%
Gln (Q)  19	  4.3%
Glu (E)  33	  7.5%
Gly (G)  17	  3.9%
His (H)  10	  2.3%
Ile (I)  25	  5.7%
Leu (L)  39	  8.9%
Lys (K)  27	  6.1%
Met (M)  14	  3.2%
Phe (F)  16	  3.6%
Pro (P)   8	  1.8%
Ser (S)  47	 10.7%
Thr (T)  30	  6.8%
Trp (W)   2	  0.5%
Tyr (Y)  18	  4.1%
Val (V)  22	  5.0%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 67
Total number of positively charged residues (Arg + Lys): 55

Atomic composition:

Carbon      C	      2197
Hydrogen    H	      3477
Nitrogen    N	       623
Oxygen      O	       720
Sulfur      S	        21

Formula: C2197H3477N623O720S21
Total number of atoms: 7038

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    38195
Abs 0.1% (=1 g/l)   0.752, assuming all pairs of Cys residues form cystines


Ext. coefficient    37820
Abs 0.1% (=1 g/l)   0.744, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 49.86
This classifies the protein as unstable.



Aliphatic index: 74.18

Grand average of hydropathicity (GRAVY): -0.706