ProtParam
User-provided sequence:
10 20 30 40 50 60
MSLSQVSPLP HIKDVVLGDT VGQGAFACVK NAHLQMDPSI ILAVKFIHVP TCKKMGLSDK
70 80 90 100 110 120
DITKEVVLQS KCSKHPNVLR LIDCNVSKEY MWIILEMADG GDLFDKIEPD VGVDSDVAQF
130 140 150 160 170 180
YFQQLVSAIN YLHVECGVAH RDIKPENILL DKNGNLKLAD FGLASQFRRK DGTLRVSMDQ
190 200 210 220 230 240
RGSPPYMAPE VLYSEEGYYA DRTDIWSIGI LLFVLLTGQT PWELPSLENE DFVFFIENDG
250 260 270 280 290 300
NLNWGPWSKI EFTHLNLLRK ILQPDPNKRV TLKALKLHPW VLRRASFSGD DGLCNDPELL
310 320 330 340 350 360
AKKLFSHLKV SLSNENYLKF TQDTNSNNRY ISTQPIGNEL AELEHDSMHF QTVSNTQRAF
370 380 390 400 410 420
TSYDSNTNYN SGTGMTQEAK WTQFISYDIA ALQFHSDEND CNELVKRHLQ FNPNKLTKFY
430 440 450 460 470 480
TLQPMDVLLP ILEKALNLSQ IRVKPDLFAN FERLCELLGY DNVFPLIINI KTKSNGGYQL
490 500 510 520
CGSISIIKIE EELKSVGFER KTGDPLEWRR LFKKISTICR DIILIPN
References and
documentation are available.
Number of amino acids: 527
Molecular weight: 60016.79
Theoretical pI: 5.86
Total number of negatively charged residues (Asp + Glu): 66
Total number of positively charged residues (Arg + Lys): 57
Atomic composition:
Carbon C 2701
Hydrogen H 4228
Nitrogen N 714
Oxygen O 792
Sulfur S 20
Formula: C
2701H
4228N
714O
792S
20
Total number of atoms: 8455
Extinction coefficients:
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 66975
Abs 0.1% (=1 g/l) 1.116, assuming all pairs of Cys residues form cystines
Ext. coefficient 66350
Abs 0.1% (=1 g/l) 1.106, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 38.25
This classifies the protein as stable.
Aliphatic index: 94.69
Grand average of hydropathicity (GRAVY): -0.265