ProtParam
User-provided sequence:
10 20 30 40 50 60
MSLSSKLSVQ DLDLKDKRVF IRVDFNVPLD GKKITSNQRI VAALPTIKYV LEHHPRYVVL
70 80 90 100 110 120
ASHLGRPNGE RNEKYSLAPV AKELQSLLGK DVTFLNDCVG PEVEAAVKAS APGSVILLEN
130 140 150 160 170 180
LRYHIEEEGS RKVDGQKVKA SKEDVQKFRH ELSSLADVYI NDAFGTAHRA HSSMVGFDLP
190 200 210 220 230 240
QRAAGFLLEK ELKYFGKALE NPTRPFLAIL GGAKVADKIQ LIDNLLDKVD SIIIGGGMAF
250 260 270 280 290 300
TFKKVLENTE IGDSIFDKAG AEIVPKLMEK AKAKGVEVVL PVDFIIADAF SADANTKTVT
310 320 330 340 350 360
DKEGIPAGWQ GLDNGPESRK LFAATVAKAK TIVWNGPPGV FEFEKFAAGT KALLDEVVKS
370 380 390 400 410
SAAGNTVIIG GGDTATVAKK YGVTDKISHV STGGGASLEL LEGKELPGVA FLSEKK
References and
documentation are available.
Number of amino acids: 416
Molecular weight: 44738.43
Theoretical pI: 7.11
Total number of negatively charged residues (Asp + Glu): 55
Total number of positively charged residues (Arg + Lys): 55
Atomic composition:
Carbon C 2014
Hydrogen H 3241
Nitrogen N 537
Oxygen O 600
Sulfur S 5
Formula: C
2014H
3241N
537O
600S
5
Total number of atoms: 6397
Extinction coefficients:
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 21430
Abs 0.1% (=1 g/l) 0.479, assuming all pairs of Cys residues form cystines
Ext. coefficient 21430
Abs 0.1% (=1 g/l) 0.479, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 23.23
This classifies the protein as stable.
Aliphatic index: 96.83
Grand average of hydropathicity (GRAVY): -0.106