ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MSLSTKREHS GDVTDSSFKR QQRSNKPSSE YTCLGHLVNL IPGKEQKVEI TNRNVTTIGR 

        70         80         90        100        110        120 
SRSCDVILSE PDISTFHAEF HLLQMDVDNF QRNLINVIDK SRNGTFINGN RLVKKDYILK 

       130        140        150        160        170        180 
NGDRIVFGKS CSFLFKYASS SSTDIENDDE KVSSESRSYK NDDEVFKKPQ ISATSSQNAT 

       190        200        210        220        230        240 
TSAAIRKLNK TRPVSFFDKY LLGKELGAGH YALVKEAKNK KTGQQVAVKI FHAQQNDDQK 

       250        260        270        280        290        300 
KNKQFREETN ILMRVQHPNI VNLLDSFVEP ISKSQIQKYL VLEKIDDGEL FERIVRKTCL 

       310        320        330        340        350        360 
RQDESKALFK QLLTGLKYLH EQNIIHRDIK PENILLNITR RENPSQVQLG PWDEDEIDIQ 

       370        380        390        400        410        420 
VKIADFGLAK FTGEMQFTNT LCGTPSYVAP EVLTKKGYTS KVDLWSAGVI LYVCLCGFPP 

       430        440        450        460        470        480 
FSDQLGPPSL KEQILQAKYA FYSPYWDKID DSVLHLISNL LVLNPDERYN IDEALNHPWF 

       490        500        510 
NDIQQQSSVS LELQRLQITD NKIPKTYSEL SCL 

References and documentation are available.

Number of amino acids: 513

Molecular weight: 58632.53

Theoretical pI: 7.96

Amino acid composition: 
Ala (A)  19	  3.7%
Arg (R)  23	  4.5%
Asn (N)  32	  6.2%
Asp (D)  35	  6.8%
Cys (C)   8	  1.6%
Gln (Q)  31	  6.0%
Glu (E)  31	  6.0%
Gly (G)  22	  4.3%
His (H)  11	  2.1%
Ile (I)  36	  7.0%
Leu (L)  51	  9.9%
Lys (K)  45	  8.8%
Met (M)   4	  0.8%
Phe (F)  23	  4.5%
Pro (P)  20	  3.9%
Ser (S)  46	  9.0%
Thr (T)  26	  5.1%
Trp (W)   4	  0.8%
Tyr (Y)  16	  3.1%
Val (V)  30	  5.8%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 66
Total number of positively charged residues (Arg + Lys): 68

Atomic composition:

Carbon      C	      2606
Hydrogen    H	      4134
Nitrogen    N	       716
Oxygen      O	       797
Sulfur      S	        12

Formula: C2606H4134N716O797S12
Total number of atoms: 8265

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    46340
Abs 0.1% (=1 g/l)   0.790, assuming all pairs of Cys residues form cystines


Ext. coefficient    45840
Abs 0.1% (=1 g/l)   0.782, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 48.57
This classifies the protein as unstable.



Aliphatic index: 86.80

Grand average of hydropathicity (GRAVY): -0.542