ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MSLTADEYKQ QGNAAFTAKD YDKAIELFTK AIEVSETPNH VLYSNRSACY TSLKKFSDAL 

        70         80         90        100        110        120 
NDANECVKIN PSWSKGYNRL GAAHLGLGDL DEAESNYKKA LELDASNKAA KEGLDQVHRT 

       130        140        150        160        170        180 
QQARQAQPDL GLTQLFADPN LIENLKKNPK TSEMMKDPQL VAKLIGYKQN PQAIGQDLFT 

       190        200        210        220        230        240 
DPRLMTIMAT LMGVDLNMDD INQSNSMPKE PETSKSTEQK KDAEPQSDST TSKENSSKAP 

       250        260        270        280        290        300 
QKEESKESEP MEVDEDDSKI EADKEKAEGN KFYKARQFDE AIEHYNKAWE LHKDITYLNN 

       310        320        330        340        350        360 
RAAAEYEKGE YETAISTLND AVEQGREMRA DYKVISKSFA RIGNAYHKLG DLKKTIEYYQ 

       370        380        390        400        410        420 
KSLTEHRTAD ILTKLRNAEK ELKKAEAEAY VNPEKAEEAR LEGKEYFTKS DWPNAVKAYT 

       430        440        450        460        470        480 
EMIKRAPEDA RGYSNRAAAL AKLMSFPEAI ADCNKAIEKD PNFVRAYIRK ATAQIAVKEY 

       490        500        510        520        530        540 
ASALETLDAA RTKDAEVNNG SSAREIDQLY YKASQQRFQP GTSNETPEET YQRAMKDPEV 

       550        560        570        580 
AAIMQDPVMQ SILQQAQQNP AALQEHMKNP EVFKKIQTLI AAGIIRTGR 

References and documentation are available.

Number of amino acids: 589

Molecular weight: 66265.10

Theoretical pI: 5.45

Amino acid composition: 
Ala (A)  74	 12.6%
Arg (R)  24	  4.1%
Asn (N)  34	  5.8%
Asp (D)  38	  6.5%
Cys (C)   3	  0.5%
Gln (Q)  33	  5.6%
Glu (E)  59	 10.0%
Gly (G)  21	  3.6%
His (H)   8	  1.4%
Ile (I)  28	  4.8%
Leu (L)  41	  7.0%
Lys (K)  60	 10.2%
Met (M)  16	  2.7%
Phe (F)  13	  2.2%
Pro (P)  24	  4.1%
Ser (S)  36	  6.1%
Thr (T)  32	  5.4%
Trp (W)   3	  0.5%
Tyr (Y)  25	  4.2%
Val (V)  17	  2.9%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 97
Total number of positively charged residues (Arg + Lys): 84

Atomic composition:

Carbon      C	      2883
Hydrogen    H	      4585
Nitrogen    N	       807
Oxygen      O	       944
Sulfur      S	        19

Formula: C2883H4585N807O944S19
Total number of atoms: 9238

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    53875
Abs 0.1% (=1 g/l)   0.813, assuming all pairs of Cys residues form cystines


Ext. coefficient    53750
Abs 0.1% (=1 g/l)   0.811, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 39.66
This classifies the protein as stable.



Aliphatic index: 66.62

Grand average of hydropathicity (GRAVY): -0.873