ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MSLTANDESP KPKKNALLKN LEIDDLIHSQ FVRSDTNGHR TTRRLFNSDA SISHRIRGSV 

        70         80         90        100        110        120 
RSDKGLNKIK KGLISQQSKL ASENSSQNIV NRDNKMGAVS FPIIEPNIEV SEELKVRIKY 

       130        140        150        160        170        180 
DSIKFFNFER LISKSSVIAP LVNKNITSSG PLIGFQRRVN RLKQTWDLAT ENMEYPYSSD 

       190        200        210        220        230        240 
NTPFRDNDSW QWYVPYGGTI KKMKDFSTKR TLPTWEDKIK FLTFLENSKS ATYINGNVSL 

       250        260        270        280        290        300 
CNHNETDQEN EDRKKRKGKV PRIKNKVWFS QIEYIVLRNY EIKPWYTSPF PEHINQNKMV 

       310        320        330        340        350        360 
FICEFCLKYM TSRYTFYRHQ LKCLTFKPPG NEIYRDGKLS VWEIDGRENV LYCQNLCLLA 

       370        380        390        400        410        420 
KCFINSKTLY YDVEPFIFYI LTEREDTENH PYQNAAKFHF VGYFSKEKFN SNDYNLSCIL 

       430        440        450        460        470        480 
TLPIYQRKGY GQFLMEFSYL LSRKESKFGT PEKPLSDLGL LTYRTFWKIK CAEVLLKLRD 

       490        500        510        520        530        540 
SARRRSNNKN EDTFQQVSLN DIAKLTGMIP TDVVFGLEQL QVLYRHKTRS LSSLDDFNYI 

       550        560        570        580        590        600 
IKIDSWNRIE NIYKTWSSKN YPRVKYDKLL WEPIILGPSF GINGMMNLEP TALADEALTN 

       610        620        630        640        650        660 
ETMAPVISNN THIENYNNSR AHNKRRRRRR RSSEHKTSKL HVNNIIEPEV PATDFFEDTV 

       670        680        690        700        710        720 
SSLTEYMCDY KNTNNDRLIY QAEKRVLESI HDRKGIPRSK FSTETHWELC FTIKNSETPL 

       730        740        750        760        770        780 
GNHAARRNDT GISSLEQDEV ENDVDTELYV GENAKEDEDE DEDFTLDDDI EDEQISEEND 

       790        800        810        820        830 
EEEDTYEEDS DDDEDGKRKG QEQDENDIES HIRKERVRKR RKITLIEDDE E 

References and documentation are available.

Number of amino acids: 831

Molecular weight: 97582.44

Theoretical pI: 6.06

Amino acid composition: 
Ala (A)  24	  2.9%
Arg (R)  55	  6.6%
Asn (N)  66	  7.9%
Asp (D)  59	  7.1%
Cys (C)  11	  1.3%
Gln (Q)  24	  2.9%
Glu (E)  75	  9.0%
Gly (G)  30	  3.6%
His (H)  17	  2.0%
Ile (I)  58	  7.0%
Leu (L)  66	  7.9%
Lys (K)  69	  8.3%
Met (M)  12	  1.4%
Phe (F)  37	  4.5%
Pro (P)  31	  3.7%
Ser (S)  67	  8.1%
Thr (T)  49	  5.9%
Trp (W)  12	  1.4%
Tyr (Y)  35	  4.2%
Val (V)  34	  4.1%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 134
Total number of positively charged residues (Arg + Lys): 124

Atomic composition:

Carbon      C	      4312
Hydrogen    H	      6723
Nitrogen    N	      1201
Oxygen      O	      1341
Sulfur      S	        23

Formula: C4312H6723N1201O1341S23
Total number of atoms: 13600

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient   118775
Abs 0.1% (=1 g/l)   1.217, assuming all pairs of Cys residues form cystines


Ext. coefficient   118150
Abs 0.1% (=1 g/l)   1.211, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 56.65
This classifies the protein as unstable.



Aliphatic index: 72.95

Grand average of hydropathicity (GRAVY): -0.853