ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MSLTDVLELN KTELFAKIRN GLPVVQRTQN LLDCKDDLLF AWHAKDSCLL VRNWRSSLAA 

        70         80         90        100        110        120 
KVNIQFQTLI PSSLVSLEVD RVLASNEGSL VALSGPRGVV IMELPRRWGP DGYYKDGKPV 

       130        140        150        160        170        180 
ITCRTFGLDT QLFLKNPHLE VRQVRWHPHS VSDSTLLVLL NNNTIRVYNH SKLRHVWQVG 

       190        200        210        220        230        240 
PPVLRSGANN SLCDFGELAV DFDIAPAAKP RVTEPETAGN NETTLDKSNK TLVAAKSLPK 

       250        260        270        280        290        300 
QERIEWPMVV LRENGNIYIL MTGVDSENTR LQGPVTITPQ AHDNYGLESC ALMIIPSLPP 

       310        320        330        340        350        360 
TIVIAESNGK LHHALLMEAE ATEHSFNEVD DSVLIEPAEY VVHVLETVEL ELGISAPATG 

       370        380        390        400        410        420 
KEGGNCPIYL KRDLINELRY FAYHNAGLHA VTVSFIAELQ RYLESESDED RLELAVSASA 

       430        440        450        460        470        480 
EYILCTKFDS SETVNAVFGL ALLQIPAGIV LLLGSGQVIS LKLVIDAQLL VTPNENKPVD 

       490        500        510        520        530        540 
SEVSQQESGP PFVDTIKSLL QRSVNQPILA DKLSSPSAQE SFELLNQAIE VLREQYLKRH 

       550        560        570        580        590        600 
DLVRAAFTRH INQIQLKKEQ QLQEIQDLEQ ERELISERAH KLAERFEEIS YNQELLVRKC 

       610        620        630        640        650        660 
NALMQRANAS LPNSVIAERE FSQEVIRLNK VTQSLAAGLE TAKKTFNKQR YHIAQSQEDL 

       670        680        690        700 
KKNAYELPEK QHRTITEILT QLTGEIDRQI TDVKRINKIV GI 

References and documentation are available.

Number of amino acids: 702

Molecular weight: 78680.85

Theoretical pI: 5.63

Amino acid composition: 
Ala (A)  51	  7.3%
Arg (R)  38	  5.4%
Asn (N)  39	  5.6%
Asp (D)  31	  4.4%
Cys (C)   8	  1.1%
Gln (Q)  38	  5.4%
Glu (E)  60	  8.5%
Gly (G)  30	  4.3%
His (H)  18	  2.6%
Ile (I)  43	  6.1%
Leu (L)  91	 13.0%
Lys (K)  36	  5.1%
Met (M)   7	  1.0%
Phe (F)  18	  2.6%
Pro (P)  32	  4.6%
Ser (S)  50	  7.1%
Thr (T)  36	  5.1%
Trp (W)   6	  0.9%
Tyr (Y)  15	  2.1%
Val (V)  55	  7.8%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 91
Total number of positively charged residues (Arg + Lys): 74

Atomic composition:

Carbon      C	      3490
Hydrogen    H	      5629
Nitrogen    N	       971
Oxygen      O	      1063
Sulfur      S	        15

Formula: C3490H5629N971O1063S15
Total number of atoms: 11168

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    55850
Abs 0.1% (=1 g/l)   0.710, assuming all pairs of Cys residues form cystines


Ext. coefficient    55350
Abs 0.1% (=1 g/l)   0.703, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 46.04
This classifies the protein as unstable.



Aliphatic index: 104.43

Grand average of hydropathicity (GRAVY): -0.234