ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MSLWVDKYRP KSLNALSHNE ELTNFLKSLS DQPRDLPHLL LYGPNGTGKK TRCMALLESI 

        70         80         90        100        110        120 
FGPGVYRLKI DVRQFVTASN RKLELNVVSS PYHLEITPSD MGNNDRIVIQ ELLKEVAQME 

       130        140        150        160        170        180 
QVDFQDSKDG LAHRYKCVII NEANSLTKDA QAALRRTMEK YSKNIRLIMV CDSMSPIIAP 

       190        200        210        220        230        240 
IKSRCLLIRC PAPSDSEIST ILSDVVTNER IQLETKDILK RIAQASNGNL RVSLLMLESM 

       250        260        270        280        290        300 
ALNNELALKS SSPIIKPDWI IVIHKLTRKI VKERSVNSLI ECRAVLYDLL AHCIPANIIL 

       310        320        330        340        350 
KELTFSLLDV ETLNTTNKSS IIEYSSVFDE RLSLGNKAIF HLEGFIAKVM CCLD 

References and documentation are available.

Number of amino acids: 354

Molecular weight: 39941.61

Theoretical pI: 8.48

Amino acid composition: 
Ala (A)  20	  5.6%
Arg (R)  20	  5.6%
Asn (N)  21	  5.9%
Asp (D)  19	  5.4%
Cys (C)   9	  2.5%
Gln (Q)   9	  2.5%
Glu (E)  22	  6.2%
Gly (G)  10	  2.8%
His (H)   7	  2.0%
Ile (I)  32	  9.0%
Leu (L)  48	 13.6%
Lys (K)  25	  7.1%
Met (M)  10	  2.8%
Phe (F)   8	  2.3%
Pro (P)  14	  4.0%
Ser (S)  34	  9.6%
Thr (T)  15	  4.2%
Trp (W)   2	  0.6%
Tyr (Y)   8	  2.3%
Val (V)  21	  5.9%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 41
Total number of positively charged residues (Arg + Lys): 45

Atomic composition:

Carbon      C	      1767
Hydrogen    H	      2909
Nitrogen    N	       485
Oxygen      O	       524
Sulfur      S	        19

Formula: C1767H2909N485O524S19
Total number of atoms: 5704

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    23420
Abs 0.1% (=1 g/l)   0.586, assuming all pairs of Cys residues form cystines


Ext. coefficient    22920
Abs 0.1% (=1 g/l)   0.574, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 39.86
This classifies the protein as stable.



Aliphatic index: 110.99

Grand average of hydropathicity (GRAVY): -0.057