ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MSMFNALNSN IEGEQYEAEE HSRELQIEQS FNILQDALID LKNKDFEKSD SKFQELFQID 

        70         80         90        100        110        120 
VVKPDRWGMY RNSSPTLDNL RYLCYRNRGM YYHLYLENNY ERLNSQELVN CILKAVENLV 

       130        140        150        160        170        180 
ESIQHSDADF AVTDLLARIF KSFNSVKLER LISEYEFTKQ ENLSLLLGRH RKFLLNDLTL 

       190        200        210        220        230        240 
MMNNYVELTN KLLVPNLSDN TIFERYHLEK YKDIKPEPLA FGPILSRISE MKKQDEEIMK 

       250        260        270        280        290        300 
KLDVFNVTLN EESWDEVAKA LKNLLPSVKT SSLIGRNMDP YNEIEEPIEA VKFELSEAIN 

       310        320        330        340        350        360 
NTPSLDRESE RQEEEQDNES VRADDKSGNL APSDIQTNEE ARPNKRTDEH IDSTKPLQRS 

       370        380        390        400        410        420 
SKRFKEREQE NSKELVMDVH KRFFGEFNTL LSYIHILPFC DFDTFASKFI IGSSDKQPEK 

       430        440        450        460        470        480 
FIPYTDLYEC LKSWSSRYTD IFNQNDYLSS GSNENEELFQ LNALLKSNAF DDKESFPRYL 

       490        500        510        520        530        540 
NDLDSDHIRS FISEVNAGNL HFHQVRLKLL FKLLGTYDEG NGRRLIIDYL WESQLLKIVL 

       550        560        570        580        590        600 
WFVFGIESNI FALINKNKRQ CKYLALSIYE LLVNHLGNIV EEITNKRIQG HKSADLKSQR 

       610        620        630        640        650        660 
NKVEKRIRSW HTLLEQIADE KDKELYVHFQ WTHYCFLQYT CDIVDSRLSE TLTSLENTIK 

       670        680        690        700        710        720 
DSDSSLDIAY PNYRHIPALN LNTVQSQKRK IRIIQNITVE DISEDTNSDT HSENHLETLE 

       730        740        750        760        770        780 
KVLLHILHPS TNHSNIDEEM VSFIFNSPFL LKIRLWGVLF SSYVKKSSIQ DVQRIYFHVL 

       790        800        810        820        830        840 
DFMKGALTSP VYKESNPHGR HQMLLTVLTA IGYLSSQLTA ILNSNRWESS DFVLEDYMFE 

       850        860        870        880        890        900 
KLLQTFFFFY TVLFYESSAV NDVSNKSFFK RASKSSGKMK DIMIDLATLI LYYYDLQAKL 

       910        920        930        940        950        960 
RTPAEQGIET TELIWSLHTL FGHFHFCDAS NGKFLDLAEK LLCQFINNDS FLQLKQILWC 

       970        980        990       1000       1010       1020 
RYHYAIASDN FSPDLHDTKA VEMEKIHSLP LGTYLIKLQY QNKNPYLSSS KTTLKQIMDN 

      1030       1040       1050       1060       1070       1080 
IIEKIGDPST LDNHIISRNS FLLNEYLSRP ITADLLKHTF SGATSLYLTS PNDELQQGMT 

      1090       1100       1110       1120       1130       1140 
AGLFYVSSLQ SLGLYKMRKK SMQARPSELD SIIRMLKNDI IYNTNRFESW ILLGKCYSYI 

      1150       1160       1170       1180       1190       1200 
VEDDLIWTSD KITVPEKKDV IALTQRKAIL CYLMAISIYY SKLDRTIDDK KIILEALDDL 

      1210       1220       1230       1240       1250       1260 
GSMLISGYYN PMNKLCFSWK SSAENTMRLS ETGEVVMEKT KKITTISDFN IEQSIFLCFN 

      1270       1280       1290       1300       1310       1320 
RACSLSGDIK SQDDVFVLNW SSFYNLAKFF FKTDGGNNCK LVAKYITQGC QIAYESSPAK 

      1330       1340       1350       1360       1370       1380 
DPIIEPHYLL VNACYKWVKR GVIGVNEALT LLSKDNQFFQ EQEEFWVNDE GLAWDYQEKF 

      1390       1400       1410       1420       1430       1440 
FFDKIIRLLR HLLSVDKKKW QHRPRYRIAR ILFDDLGDVN GALEEMDSLI SAKSINKNLV 

      1450       1460       1470       1480       1490       1500 
NIWKPDFERP GKHFIYTYQY LVLYLDLLFA IKDFNTTGLV IKKLRRFGSG TVNVNELLER 

      1510       1520       1530       1540       1550       1560 
AINVYTQSAK IKLQLQDKSY VEQILPTLNY QEFLKISEQL NQVFDQGKYP EEISSGLKLA 

      1570       1580       1590       1600       1610       1620 
FQLKKGHSGI AFDSVCLGIY FEYLYFPLAR QDQSLTDVND ENNPALPSSG SVTSKSTPDP 

      1630       1640 
TSKPSAIKKR VTKKEVFDRV RLLVDKIT 

References and documentation are available.

Number of amino acids: 1648

Molecular weight: 191680.37

Theoretical pI: 5.85

Amino acid composition: 
Ala (A)  66	  4.0%
Arg (R)  70	  4.2%
Asn (N) 106	  6.4%
Asp (D) 107	  6.5%
Cys (C)  19	  1.2%
Gln (Q)  68	  4.1%
Glu (E) 119	  7.2%
Gly (G)  54	  3.3%
His (H)  38	  2.3%
Ile (I) 119	  7.2%
Leu (L) 201	 12.2%
Lys (K) 128	  7.8%
Met (M)  28	  1.7%
Phe (F)  89	  5.4%
Pro (P)  47	  2.9%
Ser (S) 148	  9.0%
Thr (T)  76	  4.6%
Trp (W)  20	  1.2%
Tyr (Y)  73	  4.4%
Val (V)  72	  4.4%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 226
Total number of positively charged residues (Arg + Lys): 198

Atomic composition:

Carbon      C	      8647
Hydrogen    H	     13458
Nitrogen    N	      2256
Oxygen      O	      2572
Sulfur      S	        47

Formula: C8647H13458N2256O2572S47
Total number of atoms: 26980

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient   219895
Abs 0.1% (=1 g/l)   1.147, assuming all pairs of Cys residues form cystines


Ext. coefficient   218770
Abs 0.1% (=1 g/l)   1.141, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 45.22
This classifies the protein as unstable.



Aliphatic index: 92.40

Grand average of hydropathicity (GRAVY): -0.392