ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MSMHFRNKAW IILAILCFSS LIHSTEAVVT YDHKALIING QRRILISGSI HYPRSTPEMW 

        70         80         90        100        110        120 
PDLIKKAKEG GLDVIQTYVF WNGHEPSPGN YYFQDRYDLV KFTKLVHQAG LYLDLRIGPY 

       130        140        150        160        170        180 
VCAEWNFGGF PVWLKYVPGM VFRTDNEPFK IAMQKFTKKI VDMMKEEKLF ETQGGPIILS 

       190        200        210        220        230        240 
QIENEYGPMQ WEMGAAGKAY SKWTAEMALG LSTGVPWIMS KQEDAPYPII DTCNGFYCEG 

       250        260        270        280        290        300 
FKPNSDNKPK LWTENWTGWF TEFGGAIPNR PVEDIAFSVA RFIQNGGSFM NYYMYYGGTN 

       310        320        330        340        350        360 
FDRTAGVFIA TSYDYDAPID EYGLLREPKY SHLKELHKVI KLCEPALVSV DPTITSLGDK 

       370        380        390        400        410        420 
QEIHVFKSKT SCAAFLSNYD TSSAARVMFR GFPYDLPPWS VSILPDCKTE YYNTAKIRAP 

       430        440        450        460        470        480 
TILMKMIPTS TKFSWESYNE GSPSSNEAGT FVKDGLVEQI SMTRDKTDYF WYFTDITIGS 

       490        500        510        520        530        540 
DESFLKTGDN PLLTIFSAGH ALHVFVNGLL AGTSYGALSN SKLTFSQNIK LSVGINKLAL 

       550        560        570        580        590        600 
LSTAVGLPNA GVHYETWNTG ILGPVTLKGV NSGTWDMSKW KWSYKIGLRG EAMSLHTLAG 

       610        620        630        640        650        660 
SSAVKWWIKG FVVKKQPLTW YKSSFDTPRG NEPLALDMNT MGKGQVWVNG HNIGRHWPAY 

       670        680        690        700        710        720 
TARGNCGRCN YAGIYNEKKC LSHCGEPSQR WYHVPRSWLK PFGNLLVIFE EWGGDPSGIS 


LVKRTAK 

References and documentation are available.

Number of amino acids: 727

Molecular weight: 81998.87

Theoretical pI: 8.64

Amino acid composition: 
Ala (A)  43	  5.9%
Arg (R)  23	  3.2%
Asn (N)  35	  4.8%
Asp (D)  31	  4.3%
Cys (C)  11	  1.5%
Gln (Q)  16	  2.2%
Glu (E)  36	  5.0%
Gly (G)  64	  8.8%
His (H)  17	  2.3%
Ile (I)  45	  6.2%
Leu (L)  56	  7.7%
Lys (K)  52	  7.2%
Met (M)  21	  2.9%
Phe (F)  36	  5.0%
Pro (P)  40	  5.5%
Ser (S)  55	  7.6%
Thr (T)  46	  6.3%
Trp (W)  26	  3.6%
Tyr (Y)  36	  5.0%
Val (V)  38	  5.2%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 67
Total number of positively charged residues (Arg + Lys): 75

Atomic composition:

Carbon      C	      3750
Hydrogen    H	      5655
Nitrogen    N	       959
Oxygen      O	      1050
Sulfur      S	        32

Formula: C3750H5655N959O1050S32
Total number of atoms: 11446

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient   197265
Abs 0.1% (=1 g/l)   2.406, assuming all pairs of Cys residues form cystines


Ext. coefficient   196640
Abs 0.1% (=1 g/l)   2.398, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 35.67
This classifies the protein as stable.



Aliphatic index: 75.25

Grand average of hydropathicity (GRAVY): -0.260