ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MSMLPWSQIR DVSKLLLGFM LFIISIQKIA SILMSWILML RHSTIRKISF GYFFGTSIRR 

        70         80         90        100        110        120 
AFILTDFAQI YIGKITLRIG WKPGIVFHNV DLKLFGKDSH ITAHSTKDSR TYFNPRDQTF 

       130        140        150        160        170        180 
TFVINRRVLS ILKLVFSFST FFHTLALTVP NGKQYKLNIG SITISHPHDD TIKLEAFLHD 

       190        200        210        220        230        240 
FTHPETKDTL NHTGFFMVCK IGKEDDTGSN CTKVILKNWK SSLKISDVCW HLPEKKGKNL 

       250        260        270        280        290        300 
HSEPVEPFSA GDDAEMLTSY RKMLKPFHYP LKTLNILDLK VENVKLIYKK KFTIRISSAQ 

       310        320        330        340        350        360 
LYLESISILN NVSALELLPL NKPTWGDFEL SLSANAVVVD IDGNTAVRIP FGNVILTSDI 

       370        380        390        400        410        420 
LLFLLDNVPL RRTKVSSILN IINPSVFLTI HQVLEVLHLV DKFDSPETSS CTNTNDRSLN 

       430        440        450        460        470        480 
ILDLDIDRLP SFNFELLMSN FISRLHISDE ENVTFKVFST HALFSRNNLS MTPKKGQVMQ 

       490        500        510        520        530        540 
IRPDWPFAKT ALVSDQLSNY IKIVGTSLSY LRIPTEQDAN PVSIPVCGFE RLDTFLDEFS 

       550        560        570        580        590        600 
NSKLIVQSTL RHSYVSLENI EVLHTLSRAF DKIYLLISSR TKRNAAHKAN GGKLGDLNEA 

       610        620        630        640        650        660 
KKTFNWSLKL RMKDISCSLL VAGFLPKNLD PVEAENFNLS DVTRGAKVVF TESILLADSQ 

       670        680        690        700        710        720 
EKNFTIIDAS VYRFMDGTTY KPSPEVIIQF TNLLLSFNDS DEIHFSLPKI KFKMDVNIIW 

       730        740        750        760        770        780 
LWFYIRSIWI KFRPNSKLSR NSVSSVKSVN VLDRLRVDIG KMIIELTLPH NTEVLLIFER 

       790        800        810        820        830        840 
IGLSSSTKNL TIASLSAYVV SVYVKHIKVY VSLLNINDFE LDTEELICKK SAVINTSLIH 

       850        860        870        880        890        900 
FHAEYHFRFY MITDNIVTLY KSFKQIKLAF SNLNEFKRLY PQQQFPKKVP NLHICCQDFL 

       910        920        930        940        950        960 
IDIEEDPFEQ ELGLILKVGV LEQRERLKKL EEFKEKLSTY EDMNVRLRSL YDTSRGQSFF 

       970        980        990       1000       1010       1020 
PEFYANDQEY EQKAYLRLLE NFSTSWIARY RKAKLSFYGM PYRVISREEL GTKYHLFTRQ 

      1030       1040       1050       1060       1070       1080 
KTSTVANLVV KDLDFKLGSP SFPLDNYMDF VYQYGKKVPK STEYTLLIIL GLKIKSALWE 

      1090       1100       1110       1120       1130       1140 
LRLRDYPIPA ISFPDTFTTG DVVFAEKMPA PCALHTVYVP FVSSAQRSPY NDANTIYGLH 

      1150       1160       1170       1180       1190       1200 
IIRTINSVKT YFNIRSMVTS SSSARITWGK SLQPGYESLM LWFDFLTKPL IDPSKKLGFW 

      1210       1220       1230       1240       1250       1260 
DKFRYLVHGK WIYEFSEESE IHLNIKGSHD PYKITDDGAG LAFCWSGGTT IYVHNSTDPK 

      1270       1280       1290       1300       1310       1320 
EFLKIESQRF QLAVPDFAKV SKFDKVFMKL DGRVIWTLGL LFEQGDISKA GDEERFLPNR 

      1330       1340       1350       1360       1370       1380 
PHYEIQLMNP DGVADLDHHD TYKGFRTSFI HMSFGVYSSE HGSINSLYLA PYALTHFFKW 

      1390       1400       1410       1420       1430       1440 
WNLFHTYTSG PIRQGRLFTD VLQNKTKFGR SLFTIAYQLH LKRLMVTHIY RHITTQYDLE 

      1450       1460       1470       1480       1490       1500 
KDRKITFTGL KGRFDSLKID LHQKRVKLTH TNQKLNKSKP VWKFKMSRGE IDCAEADIRI 

      1510       1520       1530       1540       1550       1560 
LSTLFDQEAV KEILTSGLDG ILEDEPSRPI TPQDVEYLRE SDWYDYEDYI DLNQVPLGSS 

      1570       1580       1590       1600       1610       1620 
LPLKLEAIPL LYSPRISYFR KINDDGYVLA YPFGTEESHN CLIGKNHPEL TQEKLATERK 

      1630       1640       1650       1660       1670       1680 
REIEEQLKLL HITLSELQSN KGGGSVSGNS ERYARELKAE VAELNHRLHT VNTILSDLKI 

      1690       1700       1710       1720       1730       1740 
SETIPGGNTD GDSSSSLSDT DVNLENAPPI QNRISLLRTN TVESFVSMRK ASTMQVESTY 

      1750       1760       1770       1780       1790       1800 
DNRFMVHNIE LKIDNKIRHH LLEYASSAFE RKSMRFAVTY KSVTILKELL GNVLTGVRTS 

      1810       1820       1830       1840       1850       1860 
VEDYGSILED DLASNSEFIE HFEKLIREVP SDDFDYVDNY LFRLISPQVQ IKSDVERNAA 

      1870       1880       1890       1900       1910       1920 
VILAARDIEM GIIDIVQVYG KSGKRIPVDV DTIVETRYSA VSKDIQLFTL FKKDLEGPEG 

      1930       1940       1950       1960       1970       1980 
RFFHKNGYGS DKESDIWPPW IPLEMCFDGS LLDKHVFLKR RSMFLTYVAP NPLFFSANDT 

      1990       2000       2010       2020       2030       2040 
SAFSYDSRFR IAFPGLVLTS DCQQYCAVYA IAEDLLSFGS SLDEKVEKLS RILFTDEVRN 

      2050       2060       2070       2080       2090       2100 
NLENLDVSVV TALQERIKEL YYTRAYLKLH EPRLFMKSGQ ELTFDIQTST LKLTLLMTAI 

      2110       2120       2130       2140       2150       2160 
KKTYDRMGSG NRVIQKRLRW QVGTDELIWE LYDESKTPFV TIGLGPSTFI RSETSDGTNS 

      2170       2180       2190       2200       2210       2220 
NKVSISSLQC FNQQENPVYT ELLAPFYENS SYNKNAPMVE IFWILGPSVG GISDLQDLIV 

      2230       2240       2250       2260       2270       2280 
SLQPLIFKMD HKTSEKLMNY LFPKIEQTSI EPNSPELVPR SSTSSFFSSS PVLRHSLSNG 

      2290       2300       2310       2320       2330       2340 
SLSVYDAKDV DSWDLRSIQS KEGIKKHKGD HRKLSASLFV QPDYNINEMV KRSGTFFNVK 

      2350       2360       2370       2380       2390       2400 
SIIIRKTLMS VCYKGSHSLL TDVNNLIVRV PVLKYHNKLW SREEFFTALK RDVVRIVLQH 

      2410       2420       2430       2440       2450       2460 
LGNIIGNKFL PHKKENKKKT SMEIHRLLSP DSQNRDNSHI LEVEGHNSFY SSTHSSDIRS 

      2470       2480 
INSDETYNEN DGNGVKPFYP VTSEFSKNK 

References and documentation are available.

Number of amino acids: 2489

Molecular weight: 285903.91

Theoretical pI: 8.44

Amino acid composition: 
Ala (A)  89	  3.6%
Arg (R) 121	  4.9%
Asn (N) 127	  5.1%
Asp (D) 149	  6.0%
Cys (C)  18	  0.7%
Gln (Q)  67	  2.7%
Glu (E) 144	  5.8%
Gly (G) 107	  4.3%
His (H)  70	  2.8%
Ile (I) 184	  7.4%
Leu (L) 279	 11.2%
Lys (K) 184	  7.4%
Met (M)  43	  1.7%
Phe (F) 147	  5.9%
Pro (P) 100	  4.0%
Ser (S) 241	  9.7%
Thr (T) 148	  5.9%
Trp (W)  30	  1.2%
Tyr (Y)  92	  3.7%
Val (V) 149	  6.0%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 293
Total number of positively charged residues (Arg + Lys): 305

Atomic composition:

Carbon      C	     12978
Hydrogen    H	     20272
Nitrogen    N	      3400
Oxygen      O	      3751
Sulfur      S	        61

Formula: C12978H20272N3400O3751S61
Total number of atoms: 40462

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient   303205
Abs 0.1% (=1 g/l)   1.061, assuming all pairs of Cys residues form cystines


Ext. coefficient   302080
Abs 0.1% (=1 g/l)   1.057, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 42.01
This classifies the protein as unstable.



Aliphatic index: 93.48

Grand average of hydropathicity (GRAVY): -0.251