ProtParam
User-provided sequence:
10 20 30 40 50 60
MSMLSRRLFS TSRLAAFSKI KVKQPVVELD GDEMTRIIWD KIKKKLILPY LDVDLKYYDL
70 80 90 100 110 120
SVESRDATSD KITQDAAEAI KKYGVGIKCA TITPDEARVK EFNLHKMWKS PNGTIRNILG
130 140 150 160 170 180
GTVFREPIVI PRIPRLVPRW EKPIIIGRHA HGDQYKATDT LIPGPGSLEL VYKPSDPTTA
190 200 210 220 230 240
QPQTLKVYDY KGSGVAMAMY NTDESIEGFA HSSFKLAIDK KLNLFLSTKN TILKKYDGRF
250 260 270 280 290 300
KDIFQEVYEA QYKSKFEQLG IHYEHRLIDD MVAQMIKSKG GFIMALKNYD GDVQSDIVAQ
310 320 330 340 350 360
GFGSLGLMTS ILVTPDGKTF ESEAAHGTVT RHYRKYQKGE ETSTNSIASI FAWSRGLLKR
370 380 390 400 410 420
GELDNTPALC KFANILESAT LNTVQQDGIM TKDLALACGN NERSAYVTTE EFLDAVEKRL
QKEIKSIE
References and
documentation are available.
Number of amino acids: 428
Molecular weight: 48190.38
Theoretical pI: 8.84
Total number of negatively charged residues (Asp + Glu): 55
Total number of positively charged residues (Arg + Lys): 61
Atomic composition:
Carbon C 2158
Hydrogen H 3441
Nitrogen N 577
Oxygen O 642
Sulfur S 14
Formula: C
2158H
3441N
577O
642S
14
Total number of atoms: 6832
Extinction coefficients:
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 47455
Abs 0.1% (=1 g/l) 0.985, assuming all pairs of Cys residues form cystines
Ext. coefficient 47330
Abs 0.1% (=1 g/l) 0.982, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 33.13
This classifies the protein as stable.
Aliphatic index: 86.61
Grand average of hydropathicity (GRAVY): -0.371