ProtParam
User-provided sequence:
10 20 30 40 50 60
MSMSMGVRGL ALRSVSKTLF SQGVRCPSMV IGARYMSSTP EKQTDPKAKA NSIINAIPGN
70 80 90 100 110 120
NILTKTGVLG TSAAAVIYAI SNELYVINDE SILLLTFLGF TGLVAKYLAP AYKDFADARM
130 140 150 160 170 180
KKVSDVLNAS RNKHVEAVKD RIDSVSQLQN VAETTKVLFD VSKETVELES EAFELKQKVE
190 200 210 220 230 240
LAHEAKAVLD SWVRYEASLR QLEQRQLAKS VISRVQSELG NPKFQEKVLQ QSISEIEQLL
SKLK
References and
documentation are available.
Number of amino acids: 244
Molecular weight: 26925.08
Theoretical pI: 9.16
Total number of negatively charged residues (Asp + Glu): 27
Total number of positively charged residues (Arg + Lys): 32
Atomic composition:
Carbon C 1192
Hydrogen H 1962
Nitrogen N 326
Oxygen O 365
Sulfur S 7
Formula: C
1192H
1962N
326O
365S
7
Total number of atoms: 3852
Extinction coefficients:
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 14440
Abs 0.1% (=1 g/l) 0.536, assuming all pairs of Cys residues form cystines
Ext. coefficient 14440
Abs 0.1% (=1 g/l) 0.536, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 48.45
This classifies the protein as unstable.
Aliphatic index: 100.70
Grand average of hydropathicity (GRAVY): -0.127