ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MSMSNIVVFG GDSHPELVTK ICENLDIHPS KVELGKFSNG ETNIALRESV REKDVYIIQS 

        70         80         90        100        110        120 
GCGQVNDTFM QLLILISACK SASASRVTAV MPYLCYSRQP DIPYTAKGAP IISKPKENYT 

       130        140        150        160        170        180 
FESHPGTPVS SSLMTQRPGA ESSLKSLDSA IRSTINLENP QPIRTPNSSA TANNNFDIKK 

       190        200        210        220        230        240 
TLSFSRIPMI PGGKLQNTSN STDAGELFNA QNAGYKLWVV QAGTLIAHLL SAAGADHVIT 

       250        260        270        280        290        300 
MDLHDPQFPG FFDIPVDNLY CKPIAQNYIQ HRIPDYQDAV IVSPDAGGAK RATAIADALE 

       310        320        330        340        350        360 
LSFALIHKER RSQLLKGPPD ATLTSGGSLP VSPRPLVTTL VSSQNTTSSG ATGVAALEMK 

       370        380        390        400        410        420 
KTTSTSSTSS QSSNSSKFVQ TTMLVGDVRN KVCIIVDDLV DTSYTITRAA KLLKDQGSTK 

       430        440        450        460        470        480 
VYALITHGVF SGDALERIGQ SSIDKLIISN TVPQDRTLQY LGKDRVDVID VSCIIGEAIR 

       490 
RIHNGESISM LFEHGW 

References and documentation are available.

Number of amino acids: 496

Molecular weight: 53504.76

Theoretical pI: 6.83

Amino acid composition: 
Ala (A)  37	  7.5%
Arg (R)  20	  4.0%
Asn (N)  23	  4.6%
Asp (D)  29	  5.8%
Cys (C)   7	  1.4%
Gln (Q)  21	  4.2%
Glu (E)  18	  3.6%
Gly (G)  32	  6.5%
His (H)  11	  2.2%
Ile (I)  40	  8.1%
Leu (L)  41	  8.3%
Lys (K)  26	  5.2%
Met (M)  10	  2.0%
Phe (F)  14	  2.8%
Pro (P)  27	  5.4%
Ser (S)  57	 11.5%
Thr (T)  37	  7.5%
Trp (W)   2	  0.4%
Tyr (Y)  12	  2.4%
Val (V)  32	  6.5%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 47
Total number of positively charged residues (Arg + Lys): 46

Atomic composition:

Carbon      C	      2347
Hydrogen    H	      3780
Nitrogen    N	       650
Oxygen      O	       741
Sulfur      S	        17

Formula: C2347H3780N650O741S17
Total number of atoms: 7535

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    29255
Abs 0.1% (=1 g/l)   0.547, assuming all pairs of Cys residues form cystines


Ext. coefficient    28880
Abs 0.1% (=1 g/l)   0.540, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 34.59
This classifies the protein as stable.



Aliphatic index: 89.86

Grand average of hydropathicity (GRAVY): -0.156