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ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MSMSSTVIKF RAGVCEYNED SRLCTPIPVQ GEIEIKPNEE EELGFWDFEW RPTEKPVGRE

70 80 90 100 110 120
LDPISLILIP GETMWVPIKS SKSGRIFALV FSSNERYFFW LQEKNSGNLP LNELSAKDKE

130 140 150
IYNKMIGVLN NSSESDEEES NDEKQKAQDV DVSMQD


References and documentation are available.

Number of amino acids: 156 Molecular weight: 17902.06 Theoretical pI: 4.46
Amino acid composition: 
Ala (A) 4 2.6% Arg (R) 6 3.8% Asn (N) 10 6.4% Asp (D) 9 5.8% Cys (C) 2 1.3% Gln (Q) 5 3.2% Glu (E) 21 13.5% Gly (G) 8 5.1% His (H) 0 0.0% Ile (I) 11 7.1% Leu (L) 11 7.1% Lys (K) 11 7.1% Met (M) 5 3.2% Phe (F) 7 4.5% Pro (P) 9 5.8% Ser (S) 17 10.9% Thr (T) 4 2.6% Trp (W) 4 2.6% Tyr (Y) 3 1.9% Val (V) 9 5.8% Pyl (O) 0 0.0% Sec (U) 0 0.0% (B) 0 0.0% (Z) 0 0.0% (X) 0 0.0%
Total number of negatively charged residues (Asp + Glu): 30 Total number of positively charged residues (Arg + Lys): 17 Atomic composition: Carbon C 790 Hydrogen H 1226 Nitrogen N 204 Oxygen O 256 Sulfur S 7 Formula: C790H1226N204O256S7 Total number of atoms: 2483 Extinction coefficients: Extinction coefficients are in units of M-1 cm-1, at 280 nm measured in water. Ext. coefficient 26595 Abs 0.1% (=1 g/l) 1.486, assuming all pairs of Cys residues form cystines Ext. coefficient 26470 Abs 0.1% (=1 g/l) 1.479, assuming all Cys residues are reduced Estimated half-life: The N-terminal of the sequence considered is M (Met). The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro). >20 hours (yeast, in vivo). >10 hours (Escherichia coli, in vivo). Instability index: The instability index (II) is computed to be 57.84 This classifies the protein as unstable. Aliphatic index: 74.29 Grand average of hydropathicity (GRAVY): -0.631