ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MSMTICSNTP GAYPEIGAYN EVDKQLESSG FSSDSSLILN KPEVRQYWSS VSSHISRSGD 

        70         80         90        100        110        120 
VFTNDKEKIS SSIGEDAMDI DASPSLIEKY NSFPTRKILP EQDEFENDVE DDASSSLKEK 

       130        140        150        160        170        180 
SQGSCEIEIA SEISSEILNG TSADGNSEFH DFAEPPPSQN ESVALSFSQS NDLDFLNNPS 

       190        200        210        220        230        240 
GSGSSNDINR STSSISLPRH VSLDFNVYNS LCLTNEVTAS ESHNVAKFHL GKKNKKSLLP 

       250        260        270        280        290        300 
RWKTIEMYGE VVKKTQDIYS NFQYAQYILR VGLDTEKLHE LVKELEDESN SFSVDSLKEY 

       310        320        330        340        350        360 
LVNDAKVILK KLSAVGYPDA QYLLGDAYSS GVFGKIKNRR AFLLFSAAAK RMHIESVYRT 

       370        380        390        400        410        420 
AICYECGLGV TRNAPKAVNF LTFAATKNHP AAMYKLGVYS YHGLMGLPDD ILTKMDGYRW 

       430        440        450        460        470        480 
LRRATSMASS FVCGAPFELA NIYMTGYKDL IISDPDYAMA LYEKAAALGH TESARILEDA 

       490        500        510 
RRSGGFVSRG HPPSAQKYHK TSHEAVAAKK LI 

References and documentation are available.

Number of amino acids: 512

Molecular weight: 56578.33

Theoretical pI: 5.55

Amino acid composition: 
Ala (A)  42	  8.2%
Arg (R)  19	  3.7%
Asn (N)  27	  5.3%
Asp (D)  31	  6.1%
Cys (C)   6	  1.2%
Gln (Q)  11	  2.1%
Glu (E)  36	  7.0%
Gly (G)  29	  5.7%
His (H)  13	  2.5%
Ile (I)  28	  5.5%
Leu (L)  43	  8.4%
Lys (K)  35	  6.8%
Met (M)  11	  2.1%
Phe (F)  20	  3.9%
Pro (P)  20	  3.9%
Ser (S)  68	 13.3%
Thr (T)  20	  3.9%
Trp (W)   3	  0.6%
Tyr (Y)  24	  4.7%
Val (V)  26	  5.1%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 67
Total number of positively charged residues (Arg + Lys): 54

Atomic composition:

Carbon      C	      2495
Hydrogen    H	      3885
Nitrogen    N	       671
Oxygen      O	       797
Sulfur      S	        17

Formula: C2495H3885N671O797S17
Total number of atoms: 7865

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    52635
Abs 0.1% (=1 g/l)   0.930, assuming all pairs of Cys residues form cystines


Ext. coefficient    52260
Abs 0.1% (=1 g/l)   0.924, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 54.38
This classifies the protein as unstable.



Aliphatic index: 77.01

Grand average of hydropathicity (GRAVY): -0.412