ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MSNAALQVYG GDEVSAVVID PGSYTTNIGY SGSDFPQSIL PSVYGKYTAD EGNKKIFSEQ 

        70         80         90        100        110        120 
SIGIPRKDYE LKPIIENGLV IDWDTAQEQW QWALQNELYL NSNSGIPALL TEPVWNSTEN 

       130        140        150        160        170        180 
RKKSLEVLLE GMQFEACYLA PTSTCVSFAA GRPNCLVVDI GHDTCSVSPI VDGMTLSKST 

       190        200        210        220        230        240 
RRNFIAGKFI NHLIKKALEP KEIIPLFAIK QRKPEFIKKT FDYEVDKSLY DYANNRGFFQ 

       250        260        270        280        290        300 
ECKETLCHIC PTKTLEETKT ELSSTAKRSI ESPWNEEIVF DNETRYGFAE ELFLPKEDDI 

       310        320        330        340        350        360 
PANWPRSNSG VVKTWRNDYV PLKRTKPSGV NKSDKKVTPT EEKEQEAVSK STSPAANSAD 

       370        380        390        400        410        420 
TPNETGKRPL EEEKPPKENN ELIGLADLVY SSIMSSDVDL RATLAHNVVL TGGTSSIPGL 

       430        440        450        460        470        480 
SDRLMTELNK ILPSLKFRIL TTGHTIERQY QSWLGGSILT SLGTFHQLWV GKKEYEEVGV 


ERLLNDRFR 

References and documentation are available.

Number of amino acids: 489

Molecular weight: 54831.91

Theoretical pI: 5.34

Amino acid composition: 
Ala (A)  25	  5.1%
Arg (R)  20	  4.1%
Asn (N)  27	  5.5%
Asp (D)  24	  4.9%
Cys (C)   7	  1.4%
Gln (Q)  14	  2.9%
Glu (E)  44	  9.0%
Gly (G)  30	  6.1%
His (H)   6	  1.2%
Ile (I)  30	  6.1%
Leu (L)  44	  9.0%
Lys (K)  37	  7.6%
Met (M)   5	  1.0%
Phe (F)  17	  3.5%
Pro (P)  28	  5.7%
Ser (S)  43	  8.8%
Thr (T)  35	  7.2%
Trp (W)   9	  1.8%
Tyr (Y)  16	  3.3%
Val (V)  28	  5.7%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 68
Total number of positively charged residues (Arg + Lys): 57

Atomic composition:

Carbon      C	      2442
Hydrogen    H	      3834
Nitrogen    N	       648
Oxygen      O	       761
Sulfur      S	        12

Formula: C2442H3834N648O761S12
Total number of atoms: 7697

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    73715
Abs 0.1% (=1 g/l)   1.344, assuming all pairs of Cys residues form cystines


Ext. coefficient    73340
Abs 0.1% (=1 g/l)   1.338, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 46.04
This classifies the protein as unstable.



Aliphatic index: 80.74

Grand average of hydropathicity (GRAVY): -0.491